spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Identification of syntaxin 4 as a murine CENP-F-interacting protein. (A) A Y2H screen was conducted and described previously (Pooley et al., 2006). One of the interacting proteins with murine CENP-F (mCENP-F) was identified as syntaxin 4. The plasmid was isolated from a colony that survived on QDO media and subsequently sequenced. The resulting sequence was identified as the C-terminal 144 aa of syntaxin 4, labeled Y2HS4. The N-terminal 474 aa region of murine CENP-F (NTmCENP-F), was further characterized as being the region of CENP-F sufficient for syntaxin 4 interaction by Y2H. (B) Positive associations grew on QDO medium and exhibited blue color upon testing for β-Gal. As a positive control, growth was indicated by yeast transformed with pGBKT7-53 and pGADT7-T. The previously described interaction of NTmCENP-F with SNAP-25 was also used as a positive control (Pooley et al., 2006). The negative control with yeast co-expressing pGBTK-53 and the empty vector pGADT7 demonstrated no growth on the medium. The test interaction clearly demonstrates that NTmCENP-F does associate with the Y2HS4 portion of syntaxin 4 in yeast.





Right arrow Return to article