spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Transiently expressed NTmCENPF and syntaxin 4 colocalize at the TGN in COS-7 cells. COS-7 cells were cotransfected with NTmCENPF and GFP-syntaxin 4, and only those cells expressing both transient proteins were analyzed. (A) NTmCENPF and GFP-syntaxin 4 colocalize with the TGN marker golgin-97. (B) VAMP2 also colocalizes to a high degree with NTmCENPF and GFP-syntaxin 4. (C) VAMP3 does not demonstrate any significant redistribution to NTmCENPF–GFP-syntaxin 4 foci. (D) Rab11a, a marker of recycling endosomes, does not demonstrate noticeable colocalization. (E) Early endosomes, stained with EEA1, also show no significant redistribution to NTmCENPF–GFP-syntaxin 4 foci. Therefore, the NTmCENPF–GFP-syntaxin 4 complex is specific for localization at the TGN. NTmCENPF staining is indicated in blue, GFP-syntaxin 4 in green and the third marker, as indicated, is in red. Bar, 10 µm.





Right arrow Return to article