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Figure 6


Fig. 6. NTmCENPF expression interferes with cell coupling. (A) Cultured 3T3 fibroblasts were transfected with control GFP or NTmCENPF and GFP-SNAP-25 or NTmCENPF and GFP-syntaxin 4 and living, transfected cells were loaded with sulforhodamine101 dye (dark red cell with yellow spot). The first and second tier cells are outlined to quantify transfer; first tier cells touch the injected cell and second tier cells are those dye-transferred cells not touching the injected cell. (B) Percentage of dye spread was quantified according to methods described and the cells expressing NTmCENPF showed significantly less coupling at the second tier level.





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