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Fig. 5. The ENTH domain rescues phenotypic defects of epsin mutants. (A) DIC images of spores from the wild type (WT) and epsin mutants (epsin^-) expressing epsin:GFP, epsin_1-333:GFP, epsin_253-677:GFP, and PH:epsin_253-677:GFP. Scale bar: 5 µm. (B) Ratio of spore width:length in wild type (WT) and epsin-null mutants (epsin^-) and epsin-null mutants expressing epsin:GFP, epsin_1-333:GFP, epsin_253-677:GFP, and PH:epsin_253-677:GFP; n=50 for each cell line, error bars represent s.e.m. (C) Quantification of multinucleated cells in suspension cultures of wild-type (WT), epsin mutants (epsin^-), clathrin mutants (clathrin^-), and epsin mutants expressing epsin:GFP, epsin_1-333:GFP, epsin_253-677:GFP, and PH:epsin_253-677:GFP; n=300 for each cell line. Error bars represent s.e.m.

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