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Figure 7


Fig. 7. Quantification of EGF and Tf colocalization with EEA1. COS-7 cells expressing GFP-EEA1 were exposed to 50 ng/ml Alexa-Fluor-568-EGF (A,C) or 10 µg/ml Alexa-Fluor-568-Tf (B,C) for 5 minutes, after which fluorophores were washed and unlabeled Tf added at a concentration of 200 µg/ml (B,C). Plotted are the percentage of total EEA1 pixels colocalized with EGF (A) or Tf (B) over time. (C) Number of total pixels of each fluorophore over time, normalized to the maximum seen immediately after the wash step. (D) Percentage of Tf or EGF pixels colocalized with EEA1 in regions containing both fluorophores at indicated times after exposure to ligands. Results are from a single image set which is representative of a minimum of five independent experiments.





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