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Fig. 1. Effect of activation of J774.A1 M
on their consumption of O2 and generation of NO. Activation of M with 10 U ml-1 IFN
and 10 ng ml-1 LPS progressively inhibited mitochondrial O2 consumption and concomitantly increased the release of NO into the extracellular fluid (A), and decreased the spare respiratory capacity (B). The different components of cellular O2 consumption by untreated and SEITU-treated control M did not change throughout the experimental period. Administration of 500 µM SEITU at the time of activation prevented the decrease in cellular (A) and mitochondrial O2 consumption (not shown) and spare respiratory capacity (B). (C) The non-mitochondrial uptake of O2 was greatly enhanced after 12 hours of activation; this could be reduced by the administration of 10 µM oxyhaemoglobin or abolished by treatment with SEITU. *, Significantly different from values in non-activated cells; 
, Significantly different from IFN+LPS-treated cells (P<0.05, one way ANOVA, Tukey's post hoc test). Results are mean±s.d., n=5.
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