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First published online 30 September 2008
doi: 10.1242/jcs.029454
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Research Article |
1 Molecular Neurogenetics Unit, Department of Neurology and Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital and Program in Neuroscience, Harvard Medical School, Boston, MA 02114, USA
2 BioMEMS Resource Center, Department of Surgery and Bioengineering, Massachusetts General Hospital, Boston, MA 02114, USA
3 Department of Neurology and Center for Neurodegeneration and Experimental Therapeutics, University of Alabama, Birmingham, AL 35294, USA
4 Department of Molecular Cell Biology, Max F. Perutz Laboratories, University of Vienna, A-1030 Vienna, Austria
5 Department of Cell Biology, Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
Author for correspondence (e-mail: breakefield{at}hms.harvard.edu)
Accepted 21 July 2008
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E) in torsinA underlies most cases of the dominantly inherited movement disorder, early-onset torsion dystonia (DYT1). TorsinA, a member of the AAA+ ATPase superfamily, is located within the lumen of the nuclear envelope (NE) and endoplasmic reticulum (ER). We investigated an association between torsinA and nesprin-3, which spans the outer nuclear membrane (ONM) of the NE and links it to vimentin via plectin in fibroblasts. Mouse nesprin-3
co-immunoprecipitated with torsinA and this involved the C-terminal region of torsinA and the KASH domain of nesprin-3. This association with human nesprin-3 appeared to be stronger for torsinAE than for torsinA. TorsinA also associated with the KASH domains of nesprin-1 and -2 (SYNE1 and 2), which link to actin. In the absence of torsinA, in knockout mouse embryonic fibroblasts (MEFs), nesprin-3 was localized predominantly in the ER. Enrichment of yellow fluorescent protein (YFP)-nesprin-3 in the ER was also seen in the fibroblasts of DYT1 patients, with formation of YFP-positive globular structures enriched in torsinA, vimentin and actin. TorsinA-null MEFs had normal NE structure, but nuclear polarization and cell migration were delayed in a wound-healing assay, as compared with wild-type MEFs. These studies support a role for torsinA in dynamic interactions between the KASH domains of nesprins and their protein partners in the lumen of the NE, with torsinA influencing the localization of nesprins and associated cytoskeletal elements and affecting their role in nuclear and cell movement.
Key words: Nesprin, Dystonia, Cell migration, Nuclear polarization, DYT1, Vimentin, Actin
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; Kamm et al., 2008). Most cases of DYT1 are caused by a specific mutation (GAG) resulting in loss of a glutamic acid in the C-terminal region of torsinA (Ozelius et al., 1997). The mutant form of torsinA, torsinAE, appears to have a loss of function and to act in a dominant-negative manner to decrease function of wild-type torsinA (Goodchild et al., 2005; Dang et al., 2006; Pham et al., 2006; Hewett et al., 2008). Dystonic symptoms are believed to arise from abnormal functioning of neurons in the brain with no apparent neuronal loss (Rostasy et al., 2003; McNaught et al., 2004). Polymorphisms in the TOR1A gene (Ozelius et al., 1997) have been implicated in adult-onset focal forms of dystonia through genetic association studies (Clarimon et al., 2005; Kamm et al., 2006).
TorsinA is a member of the AAA+ (associated with a variety of cellular activities) superfamily of ATPases based on homology and configurational alignment (Ozelius et al., 1997; Neuwald et al., 1999; Lupas et al., 1997; Kock et al., 2006). These proteins typically form six-membered oligomeric complexes, usually associate with one or more additional proteins, and share Mg2+-ATP-binding domains, ATPase activity and secondary structure. AAA+ proteins mediate conformational changes in other proteins and perform a variety of functions, including correct folding of nascent proteins, unfolding of proteins for degradation, and dynamic interactions between proteins involved in signaling, membrane trafficking and organelle biogenesis/movement (for a review, see Hanson and Whiteheart, 2005).
TorsinA is predominantly localized in the contiguous lumen of the nuclear envelope (NE) and endoplasmic reticulum (ER) (Hewett et al., 2003; Kustedjo et al., 2000; Callan et al., 2007; Liu et al., 2003), and presumably interacts with transmembrane proteins concentrated in these locales. TorsinA is associated with lamin-associated polypeptide 1 (LAP1; TOR1AIP1) in the inner nuclear membrane (INM) of the NE and with LULL1 (TOR1AIP2) in the ER/NE (Goodchild and Dauer, 2005; Naismith et al., 2004), as well as with conventional kinesin light chain 1 (KLC1) (Kamm et al., 2004), vimentin and actin (Hewett et al., 2006) in the cytoplasm. The function of torsinA is unknown, but studies support a role in the structure of the NE (Goodchild et al., 2005; Naismith et al., 2004) and in the processing of proteins through the secretory pathway (Torres et al., 2004; Hewett et al., 2007; Hewett et al., 2008).
The present study was initiated based on our findings of coordinated movement of torsinA and vimentin during cellular recovery from microtubule dissociation (Hewett et al., 2006). Vimentin intermediate filaments (IFs) form a net around the NE and are involved in defining nuclear shape and mediating nuclear movement (Djabali, 1999). Cells lacking vimentin have abnormally shaped nuclei with invaginations (Sarria et al., 1994) and a reduced rate of migration as compared with control cells in wound assays (Eckes et al., 1998). Vimentin also participates in the distribution of NE membrane/LAP1-containing vesicles during mitosis (Maison et al., 1997), in the movement of the ER during cell migration (Eckes et al., 1998), and in early neurite outgrowth (Dubey et al., 2004). Loss of a nematode ortholog of torsinA, OOC-5, disrupts nuclear rotation during early embryogenesis (Basham and Rose, 2001).
The structure, shape and movement of the nucleus is maintained, at least in part, by interactions between proteins, such as the SUNs, which span the INM and interact with the IF lamins in the nucleus, and the nesprins (SYNEs), which span the outer nuclear membrane (ONM) and link to cytoskeletal elements, including IFs, microtubules and actin microfilaments, in the cytoplasm (Crisp et al., 2006; Starr and Fischer, 2005; Wilhelmsen et al., 2006). The SUN domain of INM proteins interacts with the KASH domain of nesprins in the lumenal space. Nesprin-1 and -2 (SYNE1 and 2) interact with actin microfilaments, UNC-83 (in nematodes) interacts with microtubules, and mouse nesprin-3 (the homolog of human nesprin-3) interacts with the cytolinker/plakin, plectin (Wilhelmsen et al., 2005; Wilhelmsen et al., 2006), which in turn links to IFs (Sonnenberg and Liem, 2007). Like the other nesprins, nesprin-3 is a type II transmembrane protein and contains a C-terminal KASH domain that lies within the lumenal space of the NE. Unlike nesprin-1 and -2, nesprin-3 lacks an actin-binding domain (ABD) and is therefore unable to associate with actin directly, but forms an interconnected mesh with actin microfilaments, microtubules and IFs through plakins (Wilhelmsen et al., 2006; Chang and Goldman, 2004; Herrmann et al., 2007). Recent commentaries on the links between the NE and cytoskeleton have suggested a possible role for torsinA in modulating the interaction between SUNs and nesprins within the NE lumen (Gerace, 2004; Worman and Gundersen, 2006).
In this study, we have investigated the association between torsinA in the NE and plectin/vimentin in the cytoplasm in primary cultures of torsinA-null and control mouse embryonic fibroblasts (MEFs), human skin fibroblasts from DYT1 patients and controls, mouse pup fibroblasts (MFs) and human 293T cells, using recombinant proteins, immunoprecipitation, pull-downs, immunocytochemistry and confocal microscopy. We found that torsinA associates with the C-terminal KASH domains of nesprin-3, nesprin-1 and nesprin-2. Under some conditions, the DYT1 mutant form of torsinA, torsinAE, associated more strongly with human nesprin-3 than did torsinA. TorsinA-null MEFs showed reduced polarization of nuclei relative to the centrosome, and delayed migration in wound-healing assays. These studies support a role for torsinA in modulating dynamic interactions between the KASH domain of nesprins and their binding partners in the lumen of the NE, with torsinA loss causing an enrichment of nesprin-3 in the ER.
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, plectin and vimentin) and between nesprin-3, plectin and vimentin (Wilhelmsen et al., 2005; Wilhelmsen et al., 2006). In order to evaluate whether endogenous torsinA participates in a composite complex that includes these proteins, we carried out immunoprecipitations of torsinA using lysates of wild-type MFs (Andrä et al., 2003). Immunoprecipitations were performed in RIPA buffer using antibodies to torsinA (D-M2A8 and D-MG10, 1:1) and to immunoglobulin G (anti-IgG) as a control. Proteins in lysates and precipitates were resolved by SDS-PAGE and blots probed with antibodies to plectin (300 kDa), nesprin-3 (120 kDa), vimentin (55 kDa), torsinA (37 kDa) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 36 kDa) (Fig. 1). Nesprin-3, plectin and vimentin co-immunoprecipitated with torsinA, with very little or no co-immunoprecipitation of the cytoplasmic control protein GAPDH. This demonstrates an association between endogenous torsinA, nesprin-3, plectin and vimentin in fibroblasts.
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E with human nesprin-3E in this complex. Human nesprin-3 exhibits 94.5% homology with mouse nesprin-3, and shares the plectin-binding domain (PBD) (supplementary material Fig. S1). As no anti-human-nesprin-3 antibody is available, we used a tagged version consisting of human nesprin-3 fused in-frame at the N-terminus to yellow fluorescent protein (YFP), termed YFP-nesprin-3. When this fusion protein was expressed in control primary human fibroblasts via a lentivirus vector, most fluorescence was found around the nucleus, typical of the localization of nesprin-3 described in mouse cells (Wilhelmsen et al., 2005) (Fig. 2A,D). Immunocytochemical staining of control cells expressing YFP-nesprin-3 showed a typical distribution of torsinA in the NE/ER (Fig. 2C) and of vimentin in the cytoplasm (Fig. 2E). By contrast, in DYT1 fibroblasts, YFP-nesprin-3 accumulated in globular structures, presumably within the lumen of the ER (Fig. 2B,G). These YFP+ globular structures were also enriched for torsinA (Fig. 2F), vimentin (Fig. 2H) and actin (supplementary material Fig. S2). These findings suggested that the presence of torsinAE inhibited the interaction of YFP-nesprin-3 with other binding partners in the NE, leading to an aggregation of overexpressed YFP-nesprin-3, cytoskeletal binding partners and torsinA-immunoreactive protein primarily in the ER.
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E expression cassettes. Forty-eight hours post-transfection, cell lysates were immunoprecipitated with antibodies to green fluorescent protein (GFP) (which also recognize YFP) and IgG (as a control), followed by SDS-PAGE and immunoblotting with antibodies for torsinA and GAPDH. Because ATP is involved in the interaction of AAA+ proteins with their substrates, immunoprecipitations were carried out in the presence of 1 mM ATP, non-hydrolysable ATP, or EDTA (with no added ATP) to evaluate the role of ATP in torsinA-nesprin-3 interactions. Both torsinA and torsinAE were more efficiently immunoprecipitated with YFP-nesprin-3 in the presence of ATP or non-hydrolysable ATP than of EDTA (Fig. 3A). TorsinA and torsinAE were not found to associate with IgG and showed no co-immunoprecipitation with GAPDH (Fig. 3A). In the presence of ATP, less torsinA (8±0.57%, n=3) was immunoprecipitated with YFP-nesprin-3 than torsinAE (25±0.88%, n=3, P<0.0001, two-way ANOVA). These findings suggest that the association of both torsinA and torsinAE with YFP-nesprin-3 is enhanced by ATP, and that there is a tighter association of torsinAE than of torsinA with YFP-nesprin-3.
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Our laboratory has previously shown that overexpression of torsinB, which is highly homologous to torsinA (Ozelius et al., 1997), or of torsinAE, leads to an enrichment of immunoreactive protein in the ER and perinuclear region in large inclusion bodies (Hewett et al., 2004). We evaluated the association between endogenous torsinB and YFP-nesprin-3 in 293T cells (supplementary material Fig. S3). Antibodies to torsinB were able to immunoprecipitate YFP-nesprin-3, indicating that both torsinB and torsinA can associate with nesprin-3 and thus might carry out related functions in the NE.
To evaluate whether the C-terminal region of torsinA is involved in the association with nesprin-3, 293T cells were co-transfected with YFP-nesprin-3 and torsinA, torsinAE or torsinA312-332 (Fig. 3B). Forty-eight hours post-transfection, cell lysates were immunoprecipitated with antibodies to GFP and IgG (as a control), followed by SDS-PAGE and immunoblotting with antibodies for torsinA and GAPDH. Again, torsinAE associated more tightly with YFP-nesprin-3 than did torsinA, whereas there was no association of YFP-nesprin-3 with torsinA312-332, indicating that the C-terminal region of torsinA is necessary for this association. To certify that torsinA binds specifically to nesprin-3 and not to YFP, 293T cells were also co-transfected with YFP and torsinA or torsinAE (Fig. 3C). Forty-eight hours post-transfection, cell lysates were immunoprecipitated with antibodies to torsinA followed by SDS-PAGE and immunoblotting with antibodies for torsinA and GFP. TorsinA or torsinAE did not immunoprecipitate YFP alone. Our recent results, and those by Phyllis Hanson's group (School of Medicine, Washington University in St Louis, MO; personal communication), suggest that torsinA has two independent binding domains: one in the N-terminal -β sub-domain of the AAA+ domains that binds LULL1 and LAP1 (transmembrane proteins in the INM), and one in the C-terminal -helical sub-domain that binds the KASH domain of nesprins. It remains to be tested whether torsinA can bind both types of partners simultaneously.
A difference in the binding of torsinA and torsinAE to nesprin-3 was also detected in pull-down experiments using recombinant maltose-binding protein (MBP) fusion proteins of torsinA. Both MBP-torsinA and MBP-torsinAE fusion proteins were able to pull down endogenous nesprin-3 in lysates from MFs in the presence of ATP, with MBP-torsinAE consistently pulling down 
threefold more than torsinA (3.0±0.3, n=3, P<0.003, two-way ANOVA) (Fig. 4A).
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Association of the KASH domain of nesprin-3 with torsinA
If torsinA associates with nesprin-3 it must do so through its KASH domain, which is the only portion of nesprin-3 located in the lumen of the NE, where torsinA resides. To evaluate whether torsinA binds directly to nesprin-3 KASH sequences, MBP-torsinA and MBP-torsinAE recombinant proteins were mixed 1:1 with GST or GST-KASH-3 and bound to glutathione-Sepharose TM4B beads in the presence of ATP. The mouse nesprin-3 KASH domain exhibits a high degree of homology with that of human nesprin-3 (88%) and the two proteins also share a PBD domain in the N-terminal region (supplementary material Fig. S1). Unbound material was removed by centrifugation and washing, and bound proteins resolved by SDS-PAGE and immunostained with antibodies to MBP and GST (Fig. 4B). Direct binding of wild-type and mutant torsinA fusion proteins to GST-KASH-3 was observed, but with no apparent binding differential between mutant and wild-type torsinA under these in vitro conditions.
Nesprin-3 has an alternatively spliced exon 1 variant, nesprin-3β, which retains the KASH domain that determines NE localization but is unable to associate with plectin (Wilhelmsen et al., 2005). Consistent with this, nesprin-3β also binds to torsinA (supplementary material Fig. S4).
To further evaluate the involvement of the KASH domain in the nesprin-3-torsinA complex, 293T fibroblasts were transfected with expression cassettes for GFP-nesprin-3 or GFP-nesprin-3KASH (Wilhelmsen et al., 2005). SDS-PAGE and immunoblotting of lysates of transfected cells revealed comparable expression levels of both fusion proteins 48 hours post-transfection (data not shown). Immunoprecipitation of torsinA was carried out in cell lysates, and precipitates were resolved by SDS-PAGE and subject to western blotting using antibodies to plectin, GFP, vimentin, torsinA and GAPDH. GFP-nesprin-3 showed the previously observed association with torsinA, but GFP-nesprin-3KASH did not (Fig. 5A). Similar amounts of torsinA were precipitated with torsinA antibodies from non-transfected cells (10±3%) as from cells transfected with GFP-nesprin-3 (13±5%) or GFP-nesprin-3KASH (11±5%). By contrast, 2.5-fold more plectin was immunoprecipitated from cells transfected with GFP-nesprin-3 than from non-transfected cells, and no detectable plectin was immunoprecipitated with torsinA from cells expressing GFP-nesprin-3KASH. However, levels of immunoprecipitated vimentin were essentially the same under all three conditions. This might be explained by the association of torsinA with the KASH domains of nesprin-1 and -2 (see below), and by the binding of the latter proteins to actin microfilaments with direct interaction with vimentin (Esue et al., 2006) and cross-links to IFs, such as vimentin, via plakins (Hermann et al., 2007).
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The KASH domains are highly homologous (65%) among nesprins, with nesprin-1 and -2 having an ABD domain and nesprin-3 a PBD domain (Wilhelmsen et al., 2006) (supplementary material Fig. S1). To evaluate the association of torsinA with these different KASH domains, 293T fibroblasts were transfected with expression cassettes for GFP-KASH fusion proteins derived from mouse nesprin-3 and human nesprin-1 and -2. Immunoprecipitation with torsinA antibodies and immunoblotting for GFP revealed an association of torsinA with all three KASH domains (Fig. 5B).
Effect of torsinA status on the distribution of endogenous nesprin-3
Since torsinA appears to be associated with nesprin-3 in the ONM, the absence of torsinA might affect the cellular distribution of nesprin-3. In most torsinA+/+ MEFs, nesprin-3 immunoreactivity (green) was primarily concentrated in the perinuclear region, as described for other cells (Wilhelmsen et al., 2005), as judged by co-immunocytochemistry for phosphodisulfide isomerase (PDI; red), a soluble chaperone in the lumen of the ER (Fig. 6Aa-c). By contrast, in most torsinA-/- MEFs, a substantial proportion of the nesprin-3 immunoreactivity appeared to be in the ER, as confirmed by relative co-localization with PDI (Fig. 6Ad-f). The relative proportion of nesprin-3 in the perinuclear region versus the ER proper was quantitated by evaluating co-localization coefficients for ER staining of PDI and nesprin-3 using a confocal microscope (Fig. 6B-D). TorsinA+/+ cells showed a predominantly perinuclear localization of nesprin-3 (co-localization coefficient with PDI of 14±5%, n=3), whereas in torsinA-/- cells nesprin-3 had extensive overlap with the ER (co-localization coefficient of 87±12%, n=3; P<0.0001, two-way ANOVA), indicating that interaction with torsinA is a factor in localizing nesprin-3 in the NE.
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). PKC is a serine-threonine kinase that phosphorylates vimentin at multiple sites, leading to partial filament disassembly (Eriksson et al., 2004). Plectin is also phosphorylated by PKC with a consequent weakening, but not elimination, of plectin-vimentin connections (Foisner et al., 1991). We confirmed that treatment of control primary human fibroblasts with PMA led to a concentration of vimentin immunoreactivity in the perinuclear region within 30 minutes (Fig. 7E,K), with no concomitant collapse of microfilaments or microtubules (supplementary material Fig. S5), and with rapid recovery upon removal of PMA and no loss of viability (data not shown). In addition to vimentin, torsinA and plectin also collapsed into the perinuclear region in PMA-treated fibroblasts (Fig. 7D,J). PMA-induced perinuclear vimentin collapse was also observed in torsinA+/+ MEFs, but not in torsinA-/- MEFs, consistent with a role for torsinA in maintaining nesprin-3 in the ONM and with nesprin-3 linking vimentin to the NE (data not shown).
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) and the involvement of vimentin and plectin in cell migration (Eckes et al., 1998; Andrä et al., 1998), the question remained as to the possible functions of the complex formed between torsinA, nesprin-3, plectin and vimentin in fibroblasts (see above). Ultrastructural evaluation of the apposition of the INM and ONM in the NE in torsinA-/- MEFs did not reveal any apparent abnormalities (supplementary material Fig. S6). To evaluate whether this torsinA-nesprin association was involved in cell migration, we carried out a wound-healing assay in which swaths were made in confluent monolayers of MEFs and the rate of migration of the leading edge was evaluated using both a standard assay (Gomes and Gundersen, 2006) and a new method employing microfabricated coverslips. In the latter method, cells are plated on coverslips that are partially covered by a thin polydimethylsiloxane (PDMS) membrane co-incident with micropatterned marks, and then covered with a thin layer of chrome (Fig. 8A) that does not affect cell adhesion or movement (Hunter et al., 1995). Cells were allowed to form a confluent monolayer on this surface and then a wound was initiated by removal of the PDMS membrane, made visible microscopically by the absence of chrome in the wound region. A significant delay in the migration of torsinA-/- cells was noted, with only 23±7% of the distance covered by torsinA+/+ cells at 4 hours post-wounding, 31±4% at 8 hours and 47±3% at 26 hours (n=7; P<0.0001 at all time points, two-way ANOVA) (Fig. 8B,C).
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As an initial step in cell migration, we also evaluated polarization of the nucleus relative to the wound edge by immunocytochemistry for pericentrin, as to determine the position of the centrosome relative to the nucleus (DAPI staining), as described (Gomes et al., 2005). At 3 hours post-wounding, there was a significant difference between the extent of nuclear polarization, with 85±5% of torsinA+/+ cells and only 38±7% of torsinA-/- cells (n=3; P<0.01, two-way ANOVA) showing positioning of the nucleus behind the centrosome relative to the wound edge (Fig. 9). Together, these findings suggest that torsinA is not crucial to the structure of the NE per se in fibroblasts, but does affect the positioning of the nuclei within migrating fibroblasts and the rate of movement of these cells.
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) and vimentin (Sonnenberg and Liem, 2007) in the cytoplasm. Using recombinant proteins, this association appears to involve a direct interaction between the KASH domain of nesprin-3 and the C-terminal region of torsinA. TorsinA also binds to the KASH domains of nesprin-1 and -2, which interact with cytoplasmic actin (Crisp et al., 2006; Starr and Fischer, 2005) (see Fig. 10A). TorsinA appears to play a role in the localization of nesprin-3 because in fibroblasts lacking torsinA, nesprin-3 is concentrated in the ER (Fig. 10B, diagram), whereas in wild-type cells it is localized predominantly in the NE region. Furthermore, the collapse of vimentin into the perinuclear region in response to PKC activation was abrogated in torsinA-null cells, consistent with vimentin being normally anchored to the NE by nesprin-3/plectin. The accumulation of nesprin-3 in the ER was also observed in a previous study upon overexpression of GFP-nesprin-3PPPT (nesprin-3 deleted for the SUN-binding domain) (Ketema et al., 2007), consistent with the interaction of nesprin-3 with both torsinA and SUN proteins. The dystonia-associated inactive mutant form of torsinA, torsinAE, was found under some conditions to bind more tightly to nesprin-3 than did torsinA. Thus, when YFP-nesprin-3 was overexpressed in control fibroblasts, it showed a predominantly NE localization, whereas in fibroblasts from DYT1 subjects it resulted in YFP-nesprin-3 globular structures located predominantly in the ER compartment that were enriched in torsinA, vimentin and actin (Fig. 10C). This torsinA-nesprin-cytoskeletal association does not appear to be involved in maintaining the structure of the NE in fibroblasts per se, but torsinA-null fibroblasts showed a reduced ability to position nuclei behind the centrosome in relation to the leading edge of cell migration in a wound assay, and migration itself was delayed in torsinA-null as compared with wild-type fibroblasts.
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Relationship between torsinA and the cytoskeleton
TorsinA has been hypothesized to be involved in modulating connections between transmembrane proteins in the INM, such as LAP1 and SUNs, and those in the ONM, such as nesprins, which in turn connect with cytoskeletal elements (Gerace, 2004; Worman and Gundersen, 2006). The present study supports a complex of torsinA-nesprin-3-plectin-vimentin in fibroblasts, with torsinA also associating with the KASH domains of nesprin-1 and -2 and hence presumably with complexes involving actin microfilaments. As nesprin-3 is primarily localized in the NE (Wilhelmsen et al., 2005), and torsinA is found in both the NE and ER, torsinA must have other partners in the ER. Since both SUNs (Starr and Fischer, 2005) and torsinA (this study) bind to the KASH domain of nesprins, it is hypothesized that, as an AAA+ protein, torsinA might confer a dynamic aspect to the interaction between nesprin and SUN proteins in the lumen of the NE.
Vimentin dynamics
Vimentin, a member of the large family of IF proteins, is especially abundant in fibroblasts and extends out from the nucleus to the plasma membrane in a dense filamentous network interlinked to actin filaments and microtubules through plakins (Wiche, 1998). Vimentin-knockout mice do not have an obvious phenotype (Colucci-Guyon et al., 1999), but their fibroblasts have impaired mechanical stability and contractility with poorly organized actin filaments and reduced directional migration (Eckes et al., 1998). A similar cellular phenotype was observed in plectin-knockout fibroblasts, again implicating an effect on actin dynamics (Andrä et al., 1998). Vimentin is also involved in initial neurite outgrowth from neurons (Dubey et al., 2004) and in retrograde signaling following nerve injury and regeneration (Perlson et al., 2005), with vimentin-null mice having abnormal brain morphology (Colucci-Guyon et al., 1999). Thus, changes in IF dynamics caused by loss of, or mutations in, torsinA could affect a wide range of neuronal properties, including process extension, response to injury and embryonic migration.
Involvement of torsinA in nuclear dynamics
A role for torsinA in nuclear dynamics was initially indicated by a nematode homolog, OOC-5, which has a perinuclear/ER localization and is involved in rotation of the nucleus during early embryogenesis (Basham and Rose, 2001). Other studies indicate that an E171Q mutation in the WalkerB ATP-binding domain of torsinA, which blocks ATPase activity, results in accumulation of torsinA near the NE, presumably owing to prolonged interaction with substrate(s) there and abnormalities in the structure of the NE (Naismith et al., 2004; Goodchild et al., 2005). High-level expression of torsinAE or torsinA produces whorled membrane inclusions that appear to `spin off' the NE/ER (Bragg et al., 2004; Gonzalez-Alegre and Paulson, 2004; Grundmann et al., 2007). These observations are consistent with torsinA regulating the interaction between the lumenal domains of transmembrane proteins that span the INM and ONM and are involved in NE structure.
The interaction of NE proteins with cytoskeletal elements contributes to intracellular movement of the nucleus during cell migration (Gomes et al., 2005; Wilhelmsen et al., 2006). For example, SUN1 (UNC84A) is involved in linkage between the centrosome and the NE (Malone et al., 2003) and other SUN proteins interact with nesprins, which link to actin microfilaments and IFs (Crisp et al., 2006; Ketema et al., 2007). Several components might underlie the reduced rate of cell migration in torsinA-/- MEFs, including disorientation of vimentin or actin microfilaments and/or a reduced rate of nuclear polarization. Polarization of the nucleus during cell migration is thought to be dependent primarily on actin microfilaments (Gomes et al., 2005). Nuclear polarization in torsinA-/- cells might be compromised by localization of nesprin-3 in the ER, with associated plakin-mediated interconnections between IFs and actin microfilaments (Chang and Goldman, 2004). The potential mislocalization of nesprin-1 and -2 in cells lacking torsinA was not investigated in this study, so it is not known whether the decrease in cell migration observed in torsinA-/- MEFs involves a disturbance in nesprin-1 and -2 connections to actin microfilaments (Crisp et al., 2006).
Interestingly, BPAG1 (dystonin), which is responsible for dystonic movements in a mouse model termed dystonia musculorum deformans (dt/dt) is, like plectin, a member of the plakin family of proteins that link together elements of the cytoskeleton (Sonnenberg and Liem, 2007). Recent studies show that an isozyme of BPAG1 is associated with the NE and affected neurons in BPAG1-deficient mice have neurofilament aggregates and improper placement of nuclei (Young et al., 2006). Thus, alterations in nuclear dynamics appear to be a common feature in neurons of dt/dt mice and torsinA-null or torsinAE homozygous knock-in mice (Goodchild et al., 2005), as well as in torsinA-null MEFs.
Implications for DYT1 dystonia
Although dystonia is primarily a disease of motor control, imaging studies indicate subtle alterations in microstructure and metabolism in certain regions of the brain in DYT1 patients (Asanuma et al., 2005; Carbon et al., 2008). These findings are consistent with torsinAE interfering with development and communication in select neuronal populations. Our findings that torsinAE, thought to be an inactive form of torsinA, appears to associate more strongly with nesprin-3 than does torsinA, and that loss of torsinA activity reduces nuclear polarization and cell migration, are consistent with there being abnormalities in brain development in DYT1 patients. Presumably, only a subset of neurons in the human brain, possibly those with normally high levels of torsinA and therefore more dependent on it than other cells, is compromised by low levels of torsinA function. TorsinA dependency is thought to be greatest during the developmental period when its expression is highest in the brain (Xiao et al., 2004; Vasudevan et al., 2006; Siegert et al., 2005) and neuronal migration is ongoing. Results from in situ hybridization with probes for torsinA and nesprin-3 genes in adult mice suggest extensive overlap of expression in striatum, cerebral cortex, substantia nigra and cerebellum (Allen Brain Atlas, Mouse Brain Project, Allen Institute for Brain Research, http://www.brain-map.org/welcome.do). These same brain regions have been implicated in the pathogenesis of dystonia (Eidelberg, 1998; Yokoi et al., 2008; Berardelli et al., 1998; Breakefield et al., 2008).
Conclusions
This study supports a role for torsinA in nuclear dynamics via its association with nesprins and the cytoskeleton. As an AAA+ protein, torsinA might confer a dynamic property to the ONM and INM protein interactions involved in the movement of nuclei within migrating cells. In DYT1 patients, mutant torsinA might compromise these interactions in some neurons, leading to delayed migration during development and thus reduced numbers of neurons in specific regions of the brain. This would predict a subtly altered neuronal circuitry in the brains of DYT1 subjects that contributes to an abnormal neurotransmission underlying the dystonic symptoms.
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), treated with Plasmocin (InvivoGen) to avoid mycoplasma contamination and used within the first four passages. MFs were generated from mouse pups (Andrä et al., 1998). Cells were maintained in culture as described (Hewett et al., 2007).
Vectors and constructs
Lentivirus vectors were derived from self-inactivating lentivirus (CS-CGW) (Sena-Esteves et al., 2004), in which the cDNA of interest under the control of the cytomegalovirus (CMV) immediate early promoter is followed by an internal ribosomal entry site (IRES) and a reporter cDNA (Hewett et al., 2006). Fusion constructs use a modified version lacking the IRES.
cDNAs for human torsinA and torsinAE (Ozelius et al., 1997) were cloned downstream of the CMV promoter yielding CS-CGW-TorA and CS-CGW-TorAE, respectively (Hewett et al., 2006). A C-terminal-deleted version of torsinA containing amino acids 1-311 (torsinA312-332) was amplified from the cDNA and inserted into the CFP-containing PCS-CGW backbone.
To generate YFP-human-nesprin-3, human nesprin-3 cDNA was amplified from single-stranded DNA that had been reverse-transcribed from human fibroblast (HF19) RNA. PCR was performed with Phusion Hot Start (Finnzymes) using primers upstream of the start codon (5'-GCCCCTGCAGGTGCCATGACTCAG-3') and downstream of the stop codon (5'-CTGATATGGATGCAGCTCTGCAGT-3'). Nested forward (5'-ATGACTCAGCAGCCCCAGGACGAC-3') and reverse (5'-TTAGGTGGGTGGTGGGCCATTGTA-3') primers were used to amplify just the cDNA. YFP sequences were PCR-amplified from pEYFP-C1 (Clontech) using primers containing a 5' NheI site (5'-AATGCTAGCGGTCGCCACCATGGTGAGCAAGGGCGAGGAG-3') and HindIII site (5'-CAGAAGCTTGTACAGCTCGTCCATGCC-3').
Vectors were produced by co-transfection of 293T cells with the lentivirus packaging plasmid (pCMVR8.91), envelope-coding plasmid (pVSVG) and vector construct, yielding typical titers of 1x108 transducing units/ml (Sena-Esteves et al., 2004).
For GST-KASH, the KASH domain (amino acids 910-975) of nesprin-3 was amplified by PCR using Phusion Hot Start and primers containing 5' BamHI (5'-GGGATCCATCCTCCAAAAATCGGAT-3') and 3' XhoI (5'-CATAAGCTTATGACTCAGCAGCCCCAGGAC-3) sites. After digestion with BamHI and XhoI, the sequence was inserted into the PGEX-4T1 vector in-frame with GST. GFP-KASH derived from nesprin-1 and -2 were kindly provided by Brian Burke, University of Florida.
Antibodies
Antibodies included mouse monoclonals specific to torsinA [D-M2A8 and D-M10 (Hewett et al., 2003) and MBP (New England Biolabs) and rabbit polyclonal antibodies to torsinA and torsinB [TAB1 (Hewett et al., 2003)] and specific to torsinB [TB2 (Hewett et al., 2004)], human plectin (BD Biosciences or Epitomics), mouse nesprin-3 (Wilhelmsen et al., 2005), protein disulfide isomerase (PDI; SPA-891, Stressgen), pericentrin (Covance), rabbit GFP (Molecular Probes), goat GFP (Abcam), -tubulin (Sigma-Aldrich), vimentin (Lab Vision), GAPDH (MAB374, Chemicon), pericentrin (PRB-432C, Covance), GST (Abgent), β-actin (clone AC-15, Sigma) and Alexa Fluor 488 phalloidin (Molecular Probes).
Immunocytochemistry
Immunocytochemistry was carried out as described (Hewett et al., 2006). DNA was stained with 0.1% 4',6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich). Antibody dilutions were as follows: D-M2A8 (1:100), -tubulin (1:1000), GFP (1:2000), human plectin (BD Biosciences) (1:50), nesprin-3 (1:1000), PDI (1:200), pericentrin (1:1000), vimentin (1:800), β-actin (1:500) and Alexa Fluor 488 phalloidin (1:1000) was used to stain actin.
Immunoprecipitation, pull-downs and western blots
For immunoprecipitation, total cell lysates (1x106 cells) were resuspended in 1 ml RIPA lysis buffer [150 mM NaCl, 50 mM Tris (pH 8.0), 1% NP40, 0.5% deoxycholate, 0.1% SDS (Hewett et al., 2006)]. The lysates were immunoprecipitated overnight at 4°C using specific antibodies: 10 µl D-M2A8 and D-MG10 (1:1) or 10 µl goat anti-mouse IgG (Abcam) per 500 µg lysate in 1 ml with 3 mg/ml Protein A-agarose (Roche), as described (Hewett et al., 2003). For immunoprecipitation of overexpressed protein in 293T cells, we added to the lysis buffer 1 mM ATP, or 1 mM non-hydrolysable ATP, or 1 mM EDTA (all Sigma). Proteins were resolved by electrophoresis in 10% SDS-PAGE gels, stained for protein and immunoblotted as described (Hewett et al., 2006). Antibody dilutions for western blots were as follows: TAB1 (1:200), D-M2A8 (1:100), nesprin-3 (1:1000), MBP (1:10,000), plectin (Epitomics) (1:1000), vimentin (1:2000), rabbit GFP (1:2000), goat GFP (1:2000), GAPDH (1:500) and GST (1:3000).
Purified recombinant MBP-torsinA and MBP-torsinAE expression plasmids (constructs started at amino acid 51 of torsinA) and the control MBP expression plasmid were constructed in pmal-C1-torA (Hewett et al., 2003) and used to transform Escherichia coli BL21 (New England Biolabs). Protein expression and purification were carried out as described (Hewett et al., 2003), with the addition of 1 mM ATP and 3 mM MgCl2 to the elution buffer. For MBP pull-down from the MF lysate, we also added 1 mM PMSF (Sigma-Aldrich). Purified recombinant GST-KASH from the nesprin-3 expression plasmid (derived from pGEX-4T1, Amersham Biosciences) and control GST protein from the original expression plasmid were used to transform E. coli BL21 and protein expression and purification were carried out as described in the pGEX manual. For GST or GST-KASH pull-down, 10 µg fusion protein bound to glutathione-Sepharose TM4B beads (Amersham Biosciences) was incubated with 10 µg eluted MBP-torsinA or MBP-torsinAE recombinant protein in 500 µl PBS containing 1 mM ATP and 3 mM MgCl2 for 1 hour at 4°C. Beads were collected by centrifugation (1095 g) and washed five times with 0.1% NP40 buffer [0.1% NP40, 150 mM NaCl, 50 mM Tris (pH 8.0)]. After an additional wash with PBS, bound proteins were eluted from the beads by resuspension in SDS-PAGE loading buffer and resolved by SDS-PAGE followed by immunoblotting using standard methods.
The percentage of proteins immunoprecipitated from lysates was determined by scanning the blots using a Perfection 2480/2580 scanner (Epson America Inc., Long Beach, CA) and quantifying band intensity using Quantity One 4.6.1 software (Bio-Rad).
Wound assay in culture
MEFs were plated on coverslips at 1x105 cells/well in 12-well plates in normal growth medium. Cell migration was assessed using the method of Eckes et al. (Eckes et al., 1998) or with a novel microfabrication device (see Fig. 8); the methods gave equivalent results. For the latter, a glass coverslip (Fisher Scientific) was partially covered by a 200- to 300-µm rectangular polydimethylsiloxane membrane (PDMS, Dow Corning). The membrane had one edge aligned with micropatterned marks to identify positions along the edge. The micropatterned membranes are manufactured by casting small amounts of PDMS between a micropatterned mold and a flat Mylar film. The mold was produced using standard microfabrication techniques and involves photopatterning a 200-µm layer of expoy-photoresist (SU8, MicroChem) onto a silicon wafer through a photolithography mask (Fineline Imaging). After the PDMS membrane was reversibly bonded to the glass surface by hydrophobic interaction, a thin (50 Å) layer of chrome was deposited on the glass using a sputtering machine (Kurt J. Lesker Sputtering System). The chrome layer coated both the glass and the PDMS, creating a pattern on the glass complementary to the PDMS membrane.
Confocal microscopy
A Carl Zeiss LSM 5 Pascal laser-scanning confocal microscope was used. Images were acquired using a 10x, 40x or 63x numerical aperture 1.4 PlanApo differential interference contrast (DIC) objective on an inverted microscope (Axiovert 200M, Zeiss) equipped with an LSM 510META scan head (Zeiss). Argon ion (488 nm), HeNe (543 nm) and HeNe (633 nm) lasers were used for excitation. Green, red and blue fluorescence emissions were detected through BP 505-530, 560-615 and 650 filters, respectively. The different fluorochromes were scanned sequentially using the multitracking function to avoid any bleed through among these fluorescent dyes. Brightfield images were obtained using the argon 488-nm laser. The resolution was 2048, 8 bit. We also used an Olympus FluoView 1000 confocal microscope with independent photostimulation and imaging channels, with the same lasers as above as well as a 361-nm UV laser, including a tunable two-photon source for reduced photodynamic damage. This microscope also has DIC optics and a photomultiplier collector for transmitted light imaging.
Electron microscopy
MEFs were grown as confluent monolayers on plastic coverslips, fixed in situ in 2% glutaraldehyde in 30 mM HEPES (pH 7.3), 150 mM NaCl, 2 mM CaCl2, 10 mM MgCl2. Coverslips were then washed in the same buffer without glutaraldehyde, incubated for 30 minutes in 0.5% KMnO4 in 150 mM NaCl, then dehydrated, embedded in araldite resin, sectioned and stained with uranyl acetate and lead citrate according to standard procedures. Sections were viewed in a JEOL transmission electron microscope operating at 100 kV and photographed with a charge-coupled device camera system (Advanced Microscopy Techniques).
Statistical analysis
Statistical analysis was performed using Prisma software (version 3.0). Results are expressed as mean ± s.e.m. Multiple comparison analysis of variance between the groups was performed by a two-way ANOVA test.
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Acknowledgments |
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* These authors contributed equally to this work 
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Andrä, K., Nikolic, B., Stocher, M., Drenckhahn, D. and Wiche, G. (1998). Not just scaffolding: plectin regulates actin dynamics in cultured cells. Genes Dev. 12, 3442-3451.
Andrä, K., Kornacker, I., Jörgl, A., Zörer, M., Spazierer, D., Fuchs, P., Fischer, I. and Wiche, G. (2003). Plectin-isoform-specific rescue of hemidesmosomal defects in plectin (-/-) keratinocytes. J. Invest. Dermatol. 120, 189-197.[CrossRef][Medline]
Asanuma, K., Carbon-Correll, M. and Eidelberg, D. (2005). Neuroimaging in human dystonia. J. Med. Invest. 52, 272-279.[CrossRef][Medline]
Basham, S. E. and Rose, L. S. (2001). The Caenorhabditis elegans polarity gene ooc-5 encodes a Torsin-related protein of the AAA ATPase superfamily. Development 128, 4645-4656.
Berardelli, A., Rothwell, J. C., Hallett, M., Thompson, P. D., Manfredi, M. and Marsden, C. D. (1998). The pathophysiology of primary dystonia. Brain 121, 1195-1212.
Bragg, D. C., Camp, S. M., Kaufman, C. A., Wilbur, J. D., Boston, H., Schuback, D. E., Hanson, P. I., Sena-Esteves, M. and Breakefield, X. O. (2004). Perinuclear biogenesis of mutant torsin-A inclusions in cultured cells infected with tetracycline-regulated herpes simplex virus type 1 amplicon vectors. Neuroscience 125, 651-661.[CrossRef][Medline]
Breakefield, X. O., Blood, A. J., Li, Y., Hallett, M., Hanson, P. I. and Standaert, D. G. (2008). The pathophysiologic basis of the dystonias. Nat. Rev. Neurosci. 9, 222-234.[CrossRef][Medline]
Callan, A. C., Bunning, S., Jones, O. T., High, S. and Swanton, E. (2007). Biosynthesis of the dystonia-associated AAA+ ATPase torsinA at the endoplasmic reticulum. Biochem. J. 401, 607-612.[CrossRef][Medline]
Carbon, M., Kingsley, P. B., Tang, C., Bressman, S. and Eidelberg, D. (2008). Microstructural white matter changes in primary torsion dystonia. Mov. Disord. 23, 234-239.[CrossRef][Medline]
Chang, L. and Goldman, R. D. (2004). Intermediate filaments mediate cytoskeletal crosstalk. Nat. Rev. Mol. Cell Biol. 5, 601-613.[CrossRef][Medline]
Clarimon, J., Asgeirsson, H., Singleton, A., Jakobsson, F., Hjaltason, H., Hardy, J. and Sveinbjornsdottir, S. (2005). Torsin A haplotype predisposes to idiopathic dystonia. Ann. Neurol. 57, 765-767.[CrossRef][Medline]
Colucci-Guyon, E., Gimenez, Y. R. M., Maurice, T., Babinet, C. and Privat, A. (1999). Cerebellar defect and impaired motor coordination in mice lacking vimentin. Glia 25, 33-43.[CrossRef][Medline]
Crisp, M., Liu, Q., Roux, K., Rattner, J. B., Shanahan, C., Burke, B., Stahl, P. D. and Hodzic, D. (2006). Coupling of the nucleus and cytoplasm: role of the LINC complex. J. Cell Biol. 172, 41-53.
Dang, M. T., Yokoi, F., McNaught, K. S., Jengelley, T. A., Jackson, T., Li, J. and Li, Y. (2005). Generation and characterization of Dyt1 DeltaGAG knock-in mouse as a model for early-onset dystonia. Exp. Neurol. 196, 452-463.[CrossRef][Medline]
Dang, M. T., Yokoi, F., Pence, M. A. and Li, Y. (2006). Motor deficits and hyperactivity in Dyt1 knockdown mice. Neurosci. Res. 56, 470-474.[CrossRef][Medline]
Djabali, K. (1999). Cytoskeletal proteins connecting intermediate filaments to cytoplasmic and nuclear periphery. Histol. Histopathol. 14, 501-509.[Medline]
Dubey, M., Hoda, S., Chan, W. K., Pimenta, A., Ortiz, D. D. and Shea, T. B. (2004). Reexpression of vimentin in differentiated neuroblastoma cells enhances elongation of axonal neurites. J. Neurosci. Res. 78, 245-249.[CrossRef][Medline]
Eckes, B., Dogic, D., Colucci-Guyon, E., Wang, N., Maniotis, A., Ingber, D., Merckling, A., Langa, F., Aumailley, M., Delouvee, A. et al. (1998). Impaired mechanical stability, migration and contractile capacity in vimentin-deficient fibroblasts. J. Cell Sci. 111, 245-249.
Eidelberg, D. (1998). Abnormal brain networks in DYT1 dystonia. Adv. Neurol. 78, 127-133.[Medline]
Eriksson, J. E., He, T., Trejo-Skalli, A. V., Harmala-Brasken, A. S., Hellman, J., Chou, Y. H. and Goldman, R. D. (2004). Specific in vivo phosphorylation sites determine the assembly dynamics of vimentin intermediate filaments. J. Cell Sci. 117, 919-932.
Esue, O., Carson, A. A., Tseng, Y. and Wirtz, D. (2006). A direct interaction between actin and vimentin filaments mediated by the tail domain of vimentin. J. Biol. Chem. 281, 30393-30399.
Foisner, R., Traub, P. and Wiche, G. (1991). Protein kinase A- and protein kinase C-regulated interaction of plectin with lamin B and vimentin. Proc. Natl. Acad. Sci. USA 88, 3812-3816.
Gerace, L. (2004). TorsinA and torsion dystonia: unraveling the architecture of the nuclear envelope. Proc. Natl. Acad. Sci. USA 101, 8839-8840.
Gomes, E. R. and Gundersen, G. G. (2006). Real-time centrosome reorientation during fibroblast migration. Methods Enzymol. 406, 579-592.[CrossRef][Medline]
Gomes, E. R., Jani, S. and Gundersen, G. G. (2005). Nuclear movement regulated by Cdc42, MRCK, myosin, and actin flow establishes MTOC polarization in migrating cells. Cell 121, 451-463.[CrossRef][Medline]
Gonzalez-Agosti, P. and Paulson, H. L. (2004). Aberrant cellular behavior of mutant torsinA implicates nuclear envelope dysfunction in DYT1 dystonia. J. Neurosci. 24, 2593-2601.
Goodchild, R. E. and Dauer, W. T. (2005). The AAA+ protein torsinA interacts with a conserved domain present in LAP1 and a novel ER protein. J. Cell Biol. 168, 855-862.
Goodchild, R. E., Kim, C. E. and Dauer, W. T. (2005). Loss of the dystonia-associated protein torsinA selectively disrupts the neuronal nuclear envelope. Neuron 48, 923-932.[CrossRef][Medline]
Grundmann, K., Reischmann, B., Vanhoutte, G., Hubener, J., Teismann, P., Hauser, T. K., Bonin, M., Wilbertz, J., Horn, S., Nguyen, H. P. et al. (2007). Overexpression of human wildtype torsinA and human DeltaGAG torsinA in a transgenic mouse model causes phenotypic abnormalities. Neurobiol. Dis. 27, 190-206.[CrossRef][Medline]
Hanson, P. I. and Whiteheart, S. W. (2005). AAA+ proteins: have engine, will work. Nat. Rev. Mol. Cell Biol. 6, 519-529.[CrossRef][Medline]
Herrmann, H., Bär, H., Kreplak, L., Strelkov, S. V. and Aebi, U. (2007). Intermediate filaments: from cell architecture to nanomechanics. Nat. Rev. Mol. Cell Biol. 8, 562-573.[CrossRef][Medline]
Hewett, J., Ziefer, P., Bergeron, D., Naismith, T., Boston, H., Slater, D., Wilbur, J., Schuback, D., Kamm, C., Smith, N. et al. (2003). TorsinA in PC12 cells: localization in the endoplasmic reticulum and response to stress. J. Neurosci. Res. 72, 158-168.[CrossRef][Medline]
Hewett, J. W., Kamm, C., Boston, H., Beauchamp, R., Naismith, T., Ozelius, L., Hanson, P. I., Breakefield, X. O. and Ramesh, V. (2004). TorsinB-perinuclear location and association with torsinA. J. Neurochem. 89, 1186-1194.[CrossRef][Medline]
Hewett, J. W., Zeng, J., Niland, B. P., Bragg, D. C. and Breakefield, X. O. (2006). Dystonia-causing mutant torsinA inhibits cell adhesion and neurite extension through interference with cytoskeletal dynamics. Neurobiol. Dis. 22, 98-111.[CrossRef][Medline]
Hewett, J. W., Tannous, B., Niland, B. P., Nery, F. C. and Breakefield, X. O. (2007). Mutant torsinA interferes with protein processing through the secretory pathway in DYT1 dystonia cells. Proc. Natl. Acad. Sci. USA 104, 7271-7276.
Hewett, J. W., Nery, F. C., Niland, B., Ge, P., Tan, P., Hadwiger, P., Tannous, B. A., Sah, D. W. and Breakefield, X. O. (2008). siRNA knock-down of mutant torsinA restores processing through secretory pathway in DYT1 dystonia cells. Hum. Mol. Genet. 17, 1436-1445.
Hunter, A., Archer, C. W., Walker, P. S. and Blunn, G. W. (1995). Attachment and proliferation of osteoblasts and fibroblasts on biomaterials for orthopaedic use. Biomaterials 16, 298-295.
Kamm, C., Boston, H., Hewett, J., Wilbur, J., Corey, D. P., Hanson, P. I., Ramesh, V. and Breakefield, X. O. (2004). The early onset dystonia protein torsinA interacts with kinesin light chain 1. J. Biol. Chem. 279, 19882-19892.
Kamm, C., Asmus, F., Muller, J., Mayer, P., Sharma, M., Muller, U., Bekert, S., Ehling, R., Illig, T., Wichmann, H. et al. (2006). Strong genetic evidence for association of TOR1A/TOR1B with idiopathic dystonia. Neurology 67, 1857-1859.
Kamm, C., Ozelius, L. J. and Breakefield, X. O. (2008). TorsinA and DYT1 early onset dystonia. Funct. Neurol. 61-72.
Ketema, M., Wilhelmsen, K., Kuikman, I., Janssen, H., Hodzic, D. and Sonnenberg, A. (2007). Requirements for the localization of nesprin-3 at the nuclear envelope and its interaction with plectin. J. Cell Sci. 120, 3384-3394.
Kock, N., Naismith, T. V., Boston, H. E., Ozelius, L. J., Corey, D. P., Breakefield, X. O. and Hanson, P. I. (2006). Effects of genetic variations in the dystonia protein torsinA: identification of polymorphism at residue 216 as protein modifier. Hum. Mol. Genet. 15, 1355-1364.
Kustedjo, K., Bracey, M. H. and Cravatt, B. F. (2000). TorsinA and its torsion dystonia-associated mutant forms are lumenal glycoproteins that exhibit distinct subcellular localizations. J. Biol. Chem. 275, 680-685.
Liu, Z., Zolkiewska, A. and Zolkiewski, M. (2003). Characterization of human torsinA and its dystonia-associated mutant form. Biochem. J. 374, 117-122.[CrossRef][Medline]
Lupas, A., Flanagan, J. M., Tamura, T. and Baumeister, W. (1997). Self-compartmentalizing proteases. Trends Biochem. Sci. 22, 399-404.[CrossRef][Medline]
Maison, C., Pyrpasopoulou, A., Theodoropoulos, P. A. and Georgatos, S. D. (1997). The inner nuclear membrane protein LAP1 forms a native complex with B-type lamins and partitions with spindle-associated mitotic vesicles. EMBO J. 16, 4839-4850.[CrossRef][Medline]
Malone, C. J., Misner, L., Le Bot, N., Tsai, M. C., Campbell, J. M., Ahringer, J. and White, J. G. (2003). The C. elegans hook protein, ZYG-12, mediates the essential attachment between the centrosome and nucleus. Cell 115, 825-836.[CrossRef][Medline]
McNaught, K. S., Kapustin, A., Jackson, T., Jengelley, T. A., Jnobaptiste, R., Shashidharan, P., Perl, D. P., Pasik, P. and Olanow, C. W. (2004). Brainstem pathology in DYT1 primary torsion dystonia. Ann. Neurol. 56, 540-547.[CrossRef][Medline]
Naismith, T. V., Heuser, J. E., Breakefield, X. O. and Hanson, P. I. (2004). TorsinA in the nuclear envelope. Proc. Natl. Acad. Sci. USA 101, 7612-7617.
Neuwald, A. F., Aravind, L., Spouge, J. L. and Koonin, E. V. (1999). AAA+: A class of chaperone-like ATPases associated with the assembly, operation, and disassembly of protein complexes. Genome Res. 9, 27-43.
Ozelius, L. J., Hewett, J. W., Page, C. E., Bressman, S. B., Kramer, P. L., Shalish, C., de Leon, D., Brin, M. F., Raymond, D., Corey, D. P. et al. (1997). The early-onset torsion dystonia gene (DYT1) encodes an ATP-binding protein. Nat. Genet. 17, 40-48.[CrossRef][Medline]
Perlson, E., Hanz, S., Ben-Yaakov, K., Segal-Ruder, Y., Seger, R. and Fainzilber, M. (2005). Vimentin-dependent spatial translocation of an activated MAP kinase in injured nerve. Neuron 45, 715-726.[CrossRef][Medline]
Pham, P., Frei, K. P., Woo, W. and Truong, D. D. (2006). Molecular defects of the dystonia-causing torsinA mutation. NeuroReport 17, 1725-1728.[CrossRef][Medline]
Rostasy, K., Augood, S. J., Hewett, J. W., Leung, J. C., Sasaki, H., Ozelius, L. J., Ramesh, V., Standaert, D. G., Breakefield, X. O. and Hedreen, J. C. (2003). TorsinA protein and neuropathology in early onset generalized dystonia with GAG deletion. Neurobiol. Dis. 12, 11-24.[CrossRef][Medline]
Sarria, A. J., Lieber, J. G., Nordeen, S. K. and Evans, R. M. (1994). The presence or absence of a vimentin-type intermediate filament network affects the shape of the nucleus in human SW-13 cells. J. Cell Sci. 107, 1593-1607.[Abstract]
Sena-Esteves, M., Tebbets, J. C., Steffens, S., Crombleholme, T. and Flake, A. W. (2004). Optimized large-scale production of high titer lentivirus vector pseudotypes. J. Virol. Methods 122, 131-139.[CrossRef][Medline]
Siegert, S., Bahn, E., Kramer, M. L., Schulz-Schaeffer, W. J., Hewett, J. W., Breakefield, X. O., Hedreen, J. C. and Rostasy, K. M. (2005). TorsinA expression is detectable in human infants as young as 4 weeks old. Brain Res. Dev. Brain Res. 157, 19-26.[CrossRef][Medline]
Sonnenberg, A. and Liem, R. K. (2007). Plakins in development and disease. Exp. Cell Res. 313, 2189-2203.[CrossRef][Medline]
Starr, D. A. and Fischer, J. A. (2005). KASH `n Karry: the KASH domain family of cargo-specific cytoskeletal adaptor proteins. BioEssays 27, 1136-1146.[CrossRef][Medline]
Torres, G. E., Sweeney, A. L., Beaulieu, J. M., Shashidharan, P. and Caron, M. G. (2004). Effect of torsinA on membrane proteins reveals a loss of function and a dominant-negative phenotype of the dystonia-associated DeltaE-torsinA mutant. Proc. Natl. Acad. Sci. USA 101, 15650-15655.
Vasudevan, A., Breakefield, X. O. and Bhide, P. (2006). Developmental patterns of torsinA and torsinB expression. Brain Res. 1073-1074, 139-145.
Wiche, G. (1998). Role of plectin in cytoskeleton organization and dynamics. J. Cell Sci. 111, 2477-2486.[Abstract]
Wilhelmsen, K., Litjens, S. H., Kuikman, I., Tshimbalanga, N., Janssen, H., van den Bout, I., Raymond, K. and Sonnenberg, A. (2005). Nesprin-3, a novel outer nuclear membrane protein, associates with the cytoskeletal linker protein plectin. J. Cell Biol. 171, 799-810.
Wilhelmsen, K., Ketema, M., Truong, H. and Sonnenberg, A. (2006). KASH-domain proteins in nuclear migration, anchorage and other processes. J. Cell Sci. 119, 5021-5029.
Worman, H. J. and Gundersen, G. G. (2006). Here come the SUNs: a nucleocytoskeletal missing link. Trends Cell Biol. 16, 67-69.[CrossRef][Medline]
Xiao, J., Gong, S., Zhao, Y. and LeDoux, M. S. (2004). Developmental expression of rat torsinA transcript and protein. Brain Res. Dev. Brain Res. 152, 47-60.[Medline]
Yokoi, F., Dang, M. T., Mitsui, S., Li, J. and Li, Y. (2008). Motor deficits and hyperactivity in cerebral cortex-specific Dyt1 conditional knockout mice. J. Biochem. 143, 39-47.
Young, K. G., Pinheiro, B. and Kothary, R. (2006). A Bpag1 isoform involved in cytoskeletal organization surrounding the nucleus. Exp. Cell Res. 312, 121-134.[CrossRef][Medline]
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 L. M. Giles, L. Li, and L.-S. Chin Printor, a Novel TorsinA-interacting Protein Implicated in Dystonia Pathogenesis J. Biol. Chem., August 7, 2009; 284(32): 21765 - 21775. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Argyelan, M. Carbon, M. Niethammer, A. M. Ulug, H. U. Voss, S. B. Bressman, V. Dhawan, and D. Eidelberg Cerebellothalamocortical Connectivity Regulates Penetrance in Dystonia J. Neurosci., August 5, 2009; 29(31): 9740 - 9747. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Muller The monogenic primary dystonias Brain, August 1, 2009; 132(8): 2005 - 2025. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Vander Heyden, T. V. Naismith, E. L. Snapp, D. Hodzic, and P. I. Hanson LULL1 Retargets TorsinA to the Nuclear Envelope Revealing an Activity That Is Impaired by the DYT1 Dystonia Mutation Mol. Biol. Cell, June 1, 2009; 20(11): 2661 - 2672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Starr A nuclear-envelope bridge positions nuclei and moves chromosomes J. Cell Sci., March 1, 2009; 122(5): 577 - 586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Metin, R. B. Vallee, P. Rakic, and P. G. Bhide Modes and Mishaps of Neuronal Migration in the Mammalian Brain J. Neurosci., November 12, 2008; 28(46): 11746 - 11752. [Abstract] [Full Text] [PDF]
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