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Figure 4


Fig. 4. Validation of a cblA-null strain. (A) Individual clones were first checked for gene disruption by PCR of genomic DNA and candidate clones, such as this (clone no 31), were assayed for cblA transcripts using RT-PCR (primers fwd960 5'-AAA CTC AAA GAT ATT CAG TGG TTT CAT A-3' and rev1926 5'-TGA ACA TAA TGAACA ACA ACT TAA ATG A-3'). The positive control was a random integrant from the same screen and the RNA loadings were controlled using Ig7 as in Fig. 2A. (B) A cblA– clone and Ax2 control cells at the indicated developmental stages were lysed and subjected to western transfer using the CblA antibody.





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