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Figure 6


Fig. 6. A comparison of ecmA induction and DimB nuclear import in Ax2 and cblA– cells. (A) Ax2 and cblA– cells, developed to the mound stage, were dissociated and induced with different concentrations of DIF in a monolayer assay. RNA was then extracted and ecmA concentration was evaluated by RT-PCR. (B) Mound cells were dissociated, suspended in KK2 buffer and treated with 100 nM DIF. At the indicated times, cells were fixed with 85% cold methanol and stained with anti-DimB antibody and then with Alexa-Fluor-488-conjugated secondary antibody (Molecular Probes). Samples were observed by confocal microscopy. This is a low affinity antibody. Hence the nuclear staining that is observed (white arrows) is relatively weak.





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