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Fig. 7. A comparison of inducible STATc tyrosine phosphorylation in control and cblA– cells. (A) cblA– cells and control Ax2 cells were developed by starvation in shaken suspension for 4 hours. They were then treated with 100 nM DIF and samples were removed at the indicated times. They were analysed by western transfer with a STATc anti-phosphotyrosine antibody and, as a loading control, with a general STATc antibody. (B) Quantitative comparison of four experiments, as in A, was performed. In some experiments the cblA+ control strain was Ax2 whereas in others it was a random integrant. (C) cblA– and control Ax2 cells were developed in suspension as in A. They were then treated with sorbitol at 200 mM, samples were removed after 15 minutes and analysed as in A, above. This is a typical example of three such experiments.
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