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Figure 3


Fig. 3. Unaltered FAK and SRF activity in Lkb1–/– MEFs. (A) FAK activity measured by anti-phosphotyrosine (p-Tyr) immunoblot signals from anti-FAK immunoprecipitates (Owen et al., 1999) after 50 minutes of adhesion on adhesive fibronectin (FN) plates or non-adhesive BSA-coated plates. Below are total cell extracts used for immunoprecipitations to indicate similar FAK levels between control (indicated by `C') and Lkb–/– immunoprecipitates. (B) SRE-luc activity in semiconfluent control and Lkb1–/– MEFs following a 24-hour serum starvation (0% FCS) or subsequent 3- or 15-hour treatment with serum (20% FCS) or TGFβ. Corrected luciferase values are normalized to the 0% FCS sample of control MEFs. The averages from five experiments using triplicate samples are shown, except for the 15-hour treatments, which represent averages from three experiments. Error bars indicate s.d.; *P<0.05. (C) Western blotting analysis of control and Lkb1–/– MEFs was performed with anti-moesin-P (p-Moesin), anti-PKB-P (p-PKB) and anti-SMA antibodies.





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