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Fig. 3. ANKRD11 expression enhances p53 transcriptional activity. Saos-2 (left) or HeLa (right) cells were cotransfected with p53-responsive reporter constructs encoding 17 tandem p53-REs (left), the CDKN1A promoter region (middle) or the MDM2 promoter region (right), together with pRL-TK and constructs expressing myc-p53 and ANKRD11-myc as indicated. Empty pLNCX2 vector was added to equalize the total amounts of plasmid used in various treatments. In HeLa cells, ANKRD11 expression resulted in a minimal increase in reporter activity in the absence of exogenous myc-p53. This is presumably due to ANKRD11-mediated activation of endogenous p53 in HeLa cells, because this phenomenon was not observed in the p53-null Saos-2 cell line. Data are represented as mean ± s.e.m. of triplicates. The expression of protein levels from HeLa cells transiently transfected with the CDKN1A promoter region reporter, pRL-TK, myc-p53 and ANKRD11-myc constructs as indicated was determined by western blot analysis (inset).
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