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Figure 3


Fig. 3. KazrinA overexpression affects cytoskeletal organization. (A-C,E-M) Single confocal slice images of keratinocytes transiently transfected with GFP (A; green) or HA-kazrinA (B,C,E-M; green). F-actin was detected with phalloidin (A-C,E,F; red), microtubules with a β-tubulin-specific antibody (G,H; red), and intermediate filaments with a pan keratin-specific antibody (I-M; red). (D) Immunoblot showing the proportion of F-actin (pellet; F) versus G-actin (supernatant; G) following centrifugation of lysates of cells transduced with GFP (upper panels) or kazrinA (KA; lower panels). Phalloidin was added to cell lysates as a positive control for detection of F-actin. L and M are enlargements of borders between cells 1 and 2, and cells 3 and 4, respectively (boxed with broken lines), in K. Asterisk in K marks cell with collapsed intermediate filament cytoskeleton. Bars, 40 µm (A-K), 3 µm (L,M).





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