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Figure 1


Fig. 1. Human endothelial cells express TCPTP in vitro and in vivo. (A) Immunoblotting of TCPTP, {alpha}1 and β1 integrins, and VEGFRs from HUVEC lysates. HeLa cell lysate is a positive control for expression of integrins and TCPTP, and tubulin blots serve as loading controls. Shown is a representative blot out of three, which each had similar results. (B) Immunoblotting of HUVECs and peripheral blood mononuclear cells (PBMCs, mainly consisting of T lymphocytes) isolated from different donors (numbers above the lanes) for TCPTP. (C) HUVECs grown on coverslips were stained for TCPTP in the absence (control) or presence of recombinant TCPTP (TC45). Note the specific TCPTP expression in the cytoplasm and membrane of the control HUVECs, which is competed out by pre-incubation of the antibody with the soluble target antigen. Scale bars: 10 µm. (D) Two-colour immunofluorescence stainings of human umbilical cords for TCPTP, {alpha}1 integrin and VEGFR2, and control IgGs. Individual channels and merged images with the indicated antibodies from an umbilical vein are shown. DAPI stainings reveal the nuclei of the endothelial lining. Scale bars: 20 µm. E, endothelial lining; L, lumen.





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