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Figure 1


Fig. 1. Stimulation of Frizzled-1 by Wnt3a activates p38 MAPK in F9 and HEK293 cells. (A) F9 cells stably expressing Rfz1 were treated with Wnt3a (20 ng/ml) for indicated periods of time and the lysates were assayed for p38 activation as described in Materials and Methods. The upper panel represents mean values ± s.e.m. obtained from three independent experiments; the lower panel displays representative blots probed with either anti-ATF2-P (p-ATF2), upper panel or with anti-p38 antibody (p38), lower panel. (B) F9 cells alone and F9 cells expressing either Rfz2 or Rfz1 were treated with Wnt3a for 15 minutes and the lysates were assayed for p38 activation. Representative blots of three independent experiments that proved highly reproducible are shown. (C) Confluent HEK293 cells were treated with Wnt3a for 15 minutes and the lysates were assayed for p38 activation. Representative blots of three independent experiments that proved highly reproducible are displayed. *P<0.05; **P<0.01 versus control (+Wnt3a, 0 minutes).





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