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Figure 2


Fig. 2. Suppression of G{alpha}q and G{alpha}s, but not G{alpha}o or G{alpha}11, attenuates Wnt3a-induced p38 MAPK activation. F9 cells expressing Rfz1 were treated with siRNAs specific for G{alpha}o, G{alpha}q, G{alpha}11 or G{alpha}s for 48 hours before treatment with Wnt3a for 15 minutes. p38 MAPK activity was then assayed. Upper panel displays mean values ± s.e.m. obtained from densitometer scanning of images from three independent experiments; the lower panel displays representative blots probed with anti-ATF2-P (p-ATF2), anti-p38 (p38), anti-G{alpha}o(G{alpha}o), anti-G{alpha}q (G{alpha}q), anti-G{alpha}11(G{alpha}11) and anti-G{alpha}s (G{alpha}s). *P<0.05 versus –Wnt3a control; #P<0.05 versus +Wnt3a control. The extent of knockdown of the G-protein {alpha}-subunit expression was routinely 75% or more.





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