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Figure 5


Fig. 5. Un-shed proAR that interacts with A-type lamin is truncated at the C-terminus. (A) HeLa cells transiently expressing wild type AREG were incubated with or without TPA. Cell lysates were immunoprecipitated with anti-lamin A/C mAb and analyzed by western blot using the mAb5 and mAb2. (B) HeLa cells transiently expressing AR-V5 were incubated with or without TPA. Cell lysates were immunoprecipitated with anti-lamin A/C mAb. Cell lysates (upper) and precipitated proteins (lower) were analyzed with indicated mAbs. (C) Cell lysates of HeLa cells expressing AR-V5, AR-{Delta}C1-V5 (lane 1), AR-{Delta}C2-V5 (lane 2) or AR-{Delta}C3-V5 (lane 3) were immunoprecipitated with anti-lamin A/C mAb. Cell lysates and precipitated proteins were analyzed with β-actin, anti-V5 mAb or mAb5.





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