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Figure 8


Fig. 8. Targeting of proAR to the nuclear envelope induces transient suppression of global transcription. (A,C) 5 mM Br-U was added in the presence or absence of TPA to the culture medium of HeLa cells transiently expressing AR-V5-C (A), AR-V5-{Delta}C10 or AR-V5-{Delta}C11 (C) followed by a 30 minute incubation. Cells were fixed and immunostained with anti-BrdU mAb, anti-V5 mAb and Hoechst 33342. Asterisks indicate AR-expressing cells. (B) HeLa cells transiently expressing AR-V5-C were incubated with or without TPA for 30 minutes and re-grown in fresh medium for the duration indicated. 5 mM Br-U was added to the culture medium 30 minutes prior to fixation. After fixation, Br-U incorporated in mRNA was stained with anti-BrdU mAb and fluorescent intensity in the AR-expressing cells was quantified (n=200). Statistical analysis was carried out as described in Materials and Methods. Scale bars: 5 µm.





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