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Figure 1


Fig. 1. Identification of a ligand that activates recruitment of NCoR to REV-ERB{alpha}. (A) Functional EC50 determination of the REV-ERB{alpha} ligand. Intensities of the FRET signals following addition of the compound at different concentrations are quantified and normalized to the basal level. Data are shown as mean ± s.e.m. for three independent experiments. (B) Effects of the REV-ERB{alpha} ligand on Pai1 transcription. Rat-1 cells were transiently transfected with the Pai1::Luc reporter construct. 48 hours later, cells were treated with the compound or DMSO (as control) for 1 hour or 24 hours. Cell lysates were then prepared and bioluminescence recorded using a luminometer. Four replicates were analyzed for each treatment group, and experiments were performed three times. Results are shown as mean ± s.e.m. *P<0.05, Student's t-test.





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