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Figure 1


Fig. 1. A preapical patch (PAP) containing gp135, gp114, CFTR and free of E-cadherin is formed in solid MDCK aggregates. (A,B) Localization of gp135 (A) or gp114 (B) and E-cadherin. Average frequencies of two-cell aggregates (± s.e.m.) are shown in A. (C,D) Localization of gp114 (C) or gp135 (D) and the CFTR channel in two-cell aggregates without PAP, with PAP, and with a lumen. (D) Gp135-positive vesicles (open arrowheads) and compact structure (closed arrowhead). Middle sections of deconvolved confocal Z-stacks of fixed and immunostained cell aggregates are shown. Corresponding DIC images are shown below the merged images. Scale bars: 5 µm.





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