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Figure 2


Fig. 2. Establishment of the preapical patch. (A) Time-sequence of deconvolved optical sections of an aggregate expressing mRFP-gp135 (top row) and E-cadherin-GFP (2nd row). Arrowheads delineate a gap in the E-cadherin surface (60 minutes). X-Y projections of the central region (outlined by a dashed line) are shown in the bottom row. The complete recording is available as supplementary material (Movie 1). Scale bar: 5 µm. (B) Dynamics of the normalized contact (Sc, red circles) and basal (Sb, black squares) surfaces of the aggregate shown in A. Error bars correspond to the calculated systematic segmentation error (see Materials and Methods). (C) Transmission electron micrographs of the PAP. The PAP (zone II) contains interdigitating microvilli (box 4) and is bordered by adhering plasma membranes (zone I). Magnified views of selected regions (box 1-box 5) are above the main image, which contains corresponding red and numbered rectangles. The inset in the main panel is an overview (light grey) of the two aggregate cells and the identified zones. Scale bars: 1 µm (main panel) and 200 nm (numbered boxes).





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