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Figure 4


Fig. 4. Ins(1,4,5)P3-R1 is selectively localized to the EC side of the myoendothelial junction in vivo. Immunocytochemistry on cremasteric muscles (A,C,E) and immunoblots of freshly isolated cremasteric ECs and VSMCs (cre-EC, cre-VSMC; B,D,F) were stained with Ins(1,4,5)P3-R1 (A,B), Ins(1,4,5)P3-R2 (C,D) or Ins(1,4,5)P3-R3 (E,F). (A,C,E) Red is the Ins(1,4,5)P3-R isoform and green is the autofluorescence of the internal elastic lamina. (G) Using protein quantification of antibody detection on actin bridges from in situ cremasteric arterioles (e.g. Isakson et al., 2008), only the Ins(1,4,5)P3-R1 isoform was present in significant quantities. *P<0.05. (H) At the TEM level, Ins(1,4,5)P3-R1 (10-nm gold particles) was localized to the EC side of the MEJ, but not the VSMC (insert of enlarged MEJ; H). Scale bars: 20 µm (A,C,E); 2 µm (H), 1 µm (insert in H).





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