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Fig. 7. Various vesicles are present in short flagella and in the flagellar pocket area of IFT^RNAi cell lines. (A-C) Wild-type cells. FP, flagellar pocket; BB, basal body; E, endosomes, G, Golgi complex, K, kinetoplast. The white arrow indicates the flat compartment visible close to the flagellar pocket membrane. (D) Section through the flagellar pocket of an IFT140^RNAi cell induced for 3 days. Notice the accumulation of IFT-like material in the short flagellum (IFT excess), the flagellum attachment zone (FAZ) and the presence of `double' (black arrowheads) and bent (black arrow) vesicles close to the flagellar pocket/FAZ region. (E,F) Sections through the flagellum of DHC1b^RNAi cells induced for 72 hours. Notice the presence of round or flat vesicles in the flagellar compartment (some are indicated by short white arrows). (G,H) IFT88^RNAi cells induced for 72 hours showing the flagellar pocket region where vesicles are also seen in a flagellar sleeve compartment (G) and the Golgi complex, close to which some double membrane (black arrowheads) or bent (black arrows) vesicles are frequently identified (H). (I) Quantification of sections that show a flat compartment close to the flagellar pocket membrane (black bars), at least one double membrane vesicle (`double', light grey) or at least one `cup-like' vesicle (dark grey) in the indicated cell lines. WT, n=63; DHC1b^RNAi, n=42; IFT140^RNAi, n=36; IFT88^RNAi, n=23.

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