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Figure 8


Fig. 8. The function of the flagellar pocket is altered in the absence of a normal flagellum in IFTRNAi cells. (A-D) Capture and accumulation of the lipophilic tracer FM4-64 (red) in the indicated cell lines induced for 48 hours. Cells were fixed and stained with DAPI (blue) immediately after washes. White arrows indicate non-flagellated cells. (C) Quantification of the amount of FM4-64 signal in the indicated cells. For IFT172RNAi induced samples, counts for flagellated and non-flagellated cells from the same slide are presented. FP+++, bright signal at the posterior end of the cell; FP+/–, weak signal at the posterior end; diffuse+++, bright signal through a large zone of the cell (always the posterior end for flagellated cells, more variable for non-flagellated ones); diffuse+/–, weak signal through a large zone of the cell. n=50-120.





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