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Figure 6


Fig. 6. P2X7R inhibition modifies axon growth-cone morphology in hippocampal neurons. Neurons were cultured for 3 DIV in the presence or absence (A) of the P2X7R antagonists BBG (5 µM) (B) or KN-62 (50 nM) (C). Growth-cone morphology was examined using phalloidin–Alexa-Fluor-594 and the neuronal morphology was defined with an anti-tyrosinated-{alpha}-tubulin antibody. Growth cones of control neurons presented lamellipodia and filopodia, whereas neurons treated with P2X7R antagonists developed filopodia only. Scale bar: 10 µm.





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