JCS027680 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. WT BMMs in growth medium were plated on tissue culture plastic. Immediately after addition of cells, phase-contrast images were collected every 10 minutes for 8 hours.

• Movie 2 -

  Movie 2. PAK1^−/− BMMs in growth medium were plated on tissue culture plastic. Immediately after addition of cells, phase-contrast images were collected every 10 minutes for 8 hours.

• Movie 3 -

  Movie 3. WT BMMs in growth medium were plated on to glass-bottomed tissue culture dishes. Phase contrast images were collected every 10 seconds for 30 minutes.

• Movie 4 -

  Movie 4. PAK1^−/− BMMs in growth medium were plated on to glass-bottomed tissue culture dishes. Phase contrast images were collected every 10 seconds for 30 minutes.

• Movie 5 -

  Movie 5. WT BMMs in growth medium were plated on to glass-bottomed tissue culture dishes. Phase contrast images were collected every 10 seconds for 30 minutes.

• Movie 6 -

  Movie 6. WT BMMs in growth medium containing 1 µg/ml U0126 were plated onto glass -bottomed tissue culture dishes. Phase contrast images were collected every 10 seconds for 30 minutes.