spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. PAK1 is required for lamellipodial stability during spreading. (A) WT and PAK1–/– BMMs were plated onto glass-bottomed culture dishes in growth medium and cell spreading was visualised by time-lapse microscopy. Images at the indicated time points of the movies are shown. Scale bars: 1 µm. (B) The length and the perimeter of WT and PAK1–/– lamellipodia was determined using movies as in A. Data shown are the mean ± s.e.m; n=145 and n=225 lamellipodia from WT and PAK1–/– BMMs, respectively, from six separate experiments. **P<0.01 and ***P<0.001, Student's t-test. (C) Kymographs of extending lamellipodia. The final frame from the movie used (left panels) indicates the region of kymograph production. Right panels show kymographs; the inset shows the kymograph with the membrane edge highlighted (white line). (D) Quantification of the number of lamellipodia per cell observed at the specified times in movies. Mean ± s.e.m.; n=9 cells from six separate experiments for both WT and PAK1–/– BMMs.





Right arrow Return to article