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Figure 6


Fig. 6. Unaltered distribution and expression of beaded filaments. (A-C) Immunofluorescence analysis of lens cryosections of 5-month-old VimR113C and wt transgenic mice. Sections were stained using antibodies against filensin, CP49 (both green) and Myc-tagged transgene vimentin (red). Expression and localisation of filensin and mutant vimentin is shown in (A) wt and in (B) VimR113C mice; (C) shows CP49. Merged images document unaltered expression and localisation of major lens proteins in the presence of vimentin aggregates that show no colocalisation. (D,E) Immunoblot detection of filensin and CP49 in soluble (sol.) and insoluble (insol.) lens extracts of wt and VimR113C transgenic mice. (D',E') Similar loadings of the corresponding Coomassie-stained SDS-gels. No changes in the amounts and in the processing of major lens proteins were detected. `Fragment R' filensin marks a major processing product of filensin, in agreement with previous findings (Sandilands et al., 1995b). Scale bar: 20 µm.





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