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Fig. 2. Change of mechanism in vitronectin adhesion by H249A-D262A mutations on uPAR. (A) vitronectin adhesion of cells expressing HD uPAR does not require uPAR–3β1-integrin association. H1299 and HEK293 cells expressing WT and HD uPAR were seeded on to vitronectin (2 µg/ml)-coated wells with or without 10 µg/ml mAb against integrin 3 (P1B5) or 5 (P1D6), nonimmune IgG (IgG) or 0.4 mM peptide 325 or its scrambled version, sc325. After incubation for 1 hour, cells were rinsed and adherent cells were stained with Giemsa. (B) Vitronectin adhesion of cells expressing HD uPAR is mediated by uPA–uPAR–Vβ5-integrin. uPAR-knockdown H1299 cells (shu), HEK293 non-transfected cells (ctl) and H1299 and HEK293 cells expressing WT and HD uPAR were seeded on vitronectin-coated wells with or without 10 µg/ml mAb to uPAR (ATN615, ATN617), integrin Vβ3 (LM609), Vβ5 (P1D6), 0.5 mM peptide RGD or control RAD, or 0.4 mM peptide β1P1 or its scrambled version, scβ1P1. Cells were incubated, rinsed and stained as above. The adhesion assays shown above are representative of at least three independent experiments.

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