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Figure 2


Fig. 2. Bulk endocytosis correlates with growth-cone motility. (A) Time-lapse phase-contrast imaging of two representative growth cones (3 DIV) over a 5-minute period prior to exposure to FM4-64 for 1 minute and fixation. The dye is internalized only in the more motile growth cone (bottom panels). (B) Quantification of the area of growth cones that are either positive or negative for bulk FM4-64 endocytosis (mean ± s.e.m.; n>70 growth cones per condition). (C) Quantification of growth-cone motility in growth cones that are either positive or negative for bulk FM4-64 endocytosis. The motility index (µm2/minute) was calculated by measuring the variation in growth-cone area over the course of 6 minutes as described in the Materials and Methods (mean ± s.e.m.; n=25 growth cones from three independent preparations; *P<0.01, Student's t test, endocytosis-positive vs endocytosis-negative growth cones). Bulk endocytosis is preferentially associated with more-dynamic growth cones. Scale bar: 5 µm.





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