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Figure 6


Fig. 6. The pathway of bulk membrane retrieval is distinct from the pathway of cholera-toxin internalization. A growth cone (2 DIV) that was exposed simultaneously to FM4-64 (red in the merged images) and Alexa-Fluor-488-conjugated cholera toxin subunit B (CtxB; green in the merged images) for 1 minute in KRH, washed and imaged during the following 30 minutes at 37°C. Immediately after loading (t=0), FM4-64 is internalized in large compartments, whereas CtxB is still associated with the plasma membrane and is subsequently endocytosed in punctae that do not overlap with FM4-64 labeling. Scale bar: 10 µm.





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