spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 9


Fig. 9. Plasma-membrane endocytosis in growth cones at early developmental stages is insensitive to depolarizing stimuli. (A) Uptake of FM4-64 (red in the merged image, arrowhead) in a growth cone of a 2-DIV neuron incubated with the dye for 3 minutes in KRH (left), followed by incubation with high K+ for 1 minute (middle). After depolarization, the growth cone was fixed and retrospectively stained for VAMP2 (green). In the right panel the overlay between the fluorescence and DIC images is shown. (B) Growth cone of a 2-DIV neuron expressing ECFP-VAMP2 (green in the merged image) incubated with high K+ for 1 minute in the presence of FM4-64 (red in the merged image). VAMP2-positive SVs do not internalize FM4-64 upon depolarization. Scale bar: 10 µm (A); 5.5 µm (B).





Right arrow Return to article