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Figure 1


Fig. 1. Subcellular location of PLCβ1a. (A-D) Confocal images of (A) HeLa cells expressing GFP-PLCβ1a, (B) MEF cells deficient in G{alpha}q/11 expressing GFP-PLCβ1a, (C) wild-type MEF cells expressing GFP-PLCβ1a and (D) HeLa cells expressing YFP-PLCβ1a without its CT domain. (E) TIRF image of a HeLa cell expressing GFP-PLCβ1a. (F) Fluorescence of GFP-PLCβ1a detected in TIRF mode upon stimulation with histamine (100 µM, arrow). Scale bars: 10 µm.





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