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Figure 5


Fig. 5. Bcl-xL overexpression impairs differentiation. C2C12 cells were transduced with either an adenoviral vector encoding the anti-apoptotic protein Bcl-xL (AdBcl-xL) or with the same vector without the transgene (AdNull). Bcl-xL expression was assessed by immunoblot (A) and caspase-3-like activity was assayed using DEVD-afc (B). Enzyme activity is expressed as an increase in arbitrary fluorescent units per minute per milligram of protein (AFU/min/mg). The effect of Bcl-xL expression on caspase processing (C) and on MyHC expression (D) was assessed by immunoblot and immunohistochemistry. MyHC is shown in green whereas DNA counterstaining (Hoechst 33342) is shown in blue. (E) The degree of muscle-cell differentiation was determined as before (see Materials and Methods). The histograms show mean + s.d. of three separate experiments.





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