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Figure 4


Fig. 4. Rip11/FIP5 knockdown increases colocalization of Tf with early endosomes. Mock-treated (A-C) or Rip11 siRNA1-treated (D-F) HeLa cells were incubated for 30 minutes with Tf-Alexa488 (A,D and green in C,F). Cells were then washed, fixed and stained with anti-EEA1 antibody (B,E and red in C,F). Yellow colour represents the extent of Tf and EEA1 overlap. Asterisks indicate early endosomes. Insets in C and F are higher magnification images. Numbers in C and F represent the extent of colocalization of Tf-Alexa488 with EEA1. The numbers are the mean ± s.d. from ten random cells. Scale bars: 4 µm.





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