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Fig. 6. Kif3B knockdown increases Tf uptake. (A) Mock-treated or Kif3B siRNA1-treated HeLa cells incubated at 37°C with Tf-Alexa488. Internalized Tf-Alexa488 was measured by flow cytometry. The data shown are the means of two independent experiments. (B) Mock-treated or Kif3B siRNA-treated HeLa cells were fixed with 4% paraformaldehyde and incubated with 80 µg/ml of Tf-Alexa488 in the absence of saponin. Plasma-membrane-associated Tf-Alexa488 was measured by flow cytometry. The data shown are the means ± s.d. of three independent experiments. (C) Mock-treated or Kif3B siRNA1-treated HeLa cells incubated at 37°C for 30 minutes with Tf-Alexa488. Cells were incubated with unlabeled Tf. Cell-associated Tf-Alexa488 was then measured by flow cytometry and is shown as arbitrary fluorescence units. The data shown are the mean ± s.d. of three independent experiments. *P<0.025.
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