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Figure 4


Fig. 4. Ncd transport in the mitotic spindle. (A) Median fluorescence position in FLncdVenus cycle 10 mitotic spindles versus time from early metaphase through anaphase. Distance from a point outside the pole in each half-spindle (n=14), normalized from zero to one, and averaged. Data are the mean ± s.e.m. The approximate time at which FRAP assays were performed is indicated. (B) Images from time-lapse sequences showing the equator (red line) and median fluorescence position in each half-spindle (blue lines) at the start, maximal position towards the equator, and end. Arrows (blue) in A indicate the times at which the images were acquired. Bar, 3 µm.





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