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Figure 2


Fig. 2. Expression of ephrin-B2 and {Delta}C-ephrin-B2 in HUVECs and PAE cells. (A) Bright-field (BF) imaging and GFP live imaging of mock-transduced, ephrin-B2- and {Delta}C-ephrin-B2-overexpressing HUVECs. Scale bar: 50 µm. (B) Ephrin-B2 and {Delta}C-ephrin-B2 overexpression in porcine aortic ECs. (PAEC) detected by immunoprecipitation with EphB4-Fc followed by western blotting using anti-ephrin-B2 antibody. (C) MTT-proliferation assay of transduced HUVECs assayed over a period of 96 hours. (D) Confocal microscopy analysis of ephrin-B2 expressed in ECs validating its apical expression. Scale bar: 10 µm.





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