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Figure 5


Fig. 5. Blocking of monocyte adhesion by using specific antibodies against EphBRs and ephrin-B2. (A) Pre-incubation of J774 cells with an anti-EphB2 or anti-EphB4 antibodies prior to seeding on HUVEC monolayers blocked the preferential adhesion to ephrin-B2-overexpressing HUVECs. Blocking with anti-EphB2 and anti-EphB4 antibodies further augmented the effect. (B) Pre-incubation of the HUVEC monolayer with anti-ephrin-B2 antibody prior to U937 cell seeding blocked the preferential adhesion to ephrin-B2-overexpressing HUVECs. Values are expressed as the mean ± s.d. of one out of three independent experiments with similar results (*P<0.05; **P<0.01).





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