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Fig. 1. Light and electron microscopy of A. gossypii hyphae. (A) Slow- and fast-growing hyphae. The square brackets indicate the section of the hyphae that grew for 7.5 minutes. (B) Hyphal diameters measured 5 µm behind the tip (y-axis) plotted against growth speed (x-axis). Mycelium from the border of a 3-day-old A. gossypii colony was inoculated on thin layer of AFM agar on microscopy slides at room temperature. Elongation speeds were determined as described in the Materials and Methods. (C) Surface expansion rate (SER, y-axis) plotted against growth speed (x-axis). The SER was determined as the product of hyphal circumference and growth speed. The circumference was calculated from the hyphal diameter assuming a circular hyphal profile. The broken line indicates the surface expansion rate of a (theoretical) hypha with a fixed diameter of 3.8 µm. (D) Spitzenkörper (white arrowhead) visible on AFM containing 19% gelatin. The numbers indicate time in seconds. The hyphae were covered with a cover slide and allowed to recover for 2 hours prior to imaging. Scale bars in A,D: 5 µm. (E) Transmission electron microscopy micrograph of 60 nm section of a cryofixed A. gossypii hyphal tip. The inset shows a higher magnification of the region enclosed by the rectangle. The white arrowheads depict small vesicles and the asterisk indicates a large vesicle. Scale bars: 400 nm. (F) The diameters of 140 tip-based vesicles were measured and plotted on the y-axis. The vesicle with the lowest diameter marks the left end of the x-axis, the vesicle with the biggest diameter indicates the right end.
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