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Figure 2


Fig. 2. The localization of exocyst components depends on growth speed. (A) AgExo70-GFP in hyphal tips (central planes of z-series). The white numbers indicate growth speed in µm/minute. (B) Growth speed and AgExo70-GFP localization. The dimensions of the AgExo70-GFP signals (y) were measured as shown in the sketch and plotted against growth speed in µm/minute (v). Micrographs for the data-points labeled `a', `b' and `c' in A. (C) AgSEC3-YFP expressing hypha. Growth speed is indicated in µm/minute. (D) Simplified speed/localization plot. Growth speeds are given in µm/minute on the y-axis. The two different localization patterns are shown on the x-axis. Growth speeds of hyphae that displayed a cortical AgSec3-YFP localization are shown on the left half of the plot, growth speeds of hyphae with a spherical AgSec3-YFP localization are shown on the right half. Hyphae that displayed a crescent-like AgSec3-YFP localization were not considered in this analysis. Scale bars: 5 µm.





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