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Fig. 5. Size variations of cortical AgExo70-GFP caps. (A) A z-series of AgEXO70-GFP hypha that grew faster than 1.50 µm/minute was subjected to blind deconvolution. Maximum projections of central planes are shown. Red in the false-colored image indicates high GFP-fluorescence intensity, blue and purple indicate low intensity. Eighty-seven percent of the hyphae showed an accumulation of AgExo70-GFP at the cortex (arrowhead, n=31). (B) The sizes of the cortical AgExo70-GFP areas were estimated assuming that the arc-shaped fluorescent zone visible in the central plane represents the central section of a spherical cap. These values (y-axis) were plotted against growth speed. (C) Frames from time-lapse Movie 1 of an AgEXO70-GFP hypha. The broken lines indicate the hyphal tip, the arrowheads indicate the border of the vesicle fusion zone. Scale bars for A,C: 5 µm. (D) The area of the vesicle fusion zone was estimated for every frame of Movie 1 and plotted against time. The red line results from plotting the mean vesicle fusion area for every 4-second interval. The kymograph shows GFP fluorescence enclosed by the red rectangle in D over time. The advancing tip front is indicated with a broken line. The constant slope of this line indicates a constant growth speed. Scale bars: 2 µm. (E) Model for the size variations of cortical AgExo70-GFP caps.

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