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Figure 6


Fig. 6. NMJ-capping cells also spread in paralysed and atrophic muscles. (A,B) Profusion of 2166 immunoreactivity (green) is seen within 1 day of injection of a locally paralysing dose of botulinum toxin type-A (Botox) but terminal Schwann-cell reactivity and sprouting, assessed by nestin immunostaining (red), is not observed until after least 6 days of paralysis in TS muscles. (C,D) Similar profusion and spread of NMJ-capping cells is observed in constitutively atrophic muscles of 15-week-old R6/2 transgenic mice. Littermate controls show normal, endplate-localised staining and distribution of NMJ-capping cells. Scale bar: 50 µm.





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