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Figure 1


Fig. 1. Rad9 accumulates in nuclear foci in response to genotoxic stress. (A) HeLa and Swiss 3T3 cells were left untreated, exposed to UV, or treated with aphidicolin. Rad9 was visualised by immunofluorescence. (B) Western blot analysis of untransfected U2OS cells or different U2OS clones stably expressing GFP-Rad9 (II-5 and II-19), using the indicated antibodies. (C) U2OS cells or clones expressing GFP-Rad9 (II-5 and II-19) were lysed and GFP-Rad9 immunoprecipitated using anti-GFP antibodies. The presence of associated proteins was analysed by immunoblotting with the indicated antibodies. (D) U2OS cells or cells expressing GFP-Rad9 were either left untreated or exposed to UV. After 1 hour, cells were lysed, and western blot analysis was performed using the indicated antibodies. (E) U2OS cells expressing GFP-Rad9 were left untreated, exposed to UV, or treated with aphidicolin. GFP-Rad9 was visualised by direct fluorescence. (F) U2OS cells expressing GFP-Rad9 were left untreated or exposed to UV. GFP-Rad9 was detected by direct fluorescence and RPA (p34 subunit) by immunofluorescence.





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