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Figure 10


Fig. 10. Effect of IGF-1 on myoblast migration, Mn2+ entry and calpain activity of control C2C12 myoblasts. (A) Wound-healing migration assay of C2C12 myoblasts in control conditions or in the presence of 5 nM IGF-1, +2 µM GsMTx4 toxin or +50 µM Z-Leu-Leu-CHO. (B) Rate of quenching of Fura-PE3 by Mn2+ (used to estimate the influx of Ca2+) in control conditions or after stimulation for 10 minutes, 1 hour or 4 hours with 5 nM IGF-1. Statistical analysis: one-way ANOVA followed by Student-Newmann-Keuls test, ***P<0.001 and ** P<0.01 compared with control conditions (n=5-14). (C) Calpain activity measured in control conditions or after stimulation for 10 minutes, 1 hour or 4 hours with 5 nM IGF-1. Statistical analysis: one way ANOVA followed by Student-Newmann-Keuls test, * P<0.05 vs control (n=6-15)





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