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Figure 4


Fig. 4. Involvement of Ca2+ channels and calpain in the migration of myoblasts. (A) Wound-healing migration assay of C2C12 myoblasts. Some cells were scraped off with a pipette tip to obtain a 600-µm-wide acellular area (visualized in DIC microscopy, panel a). 15 hours later, cells that had migrated into the acellular area were stained and counted. Cells were migrated in control conditions (b), or in the presence of 2 µM GsMTx4 toxin (c) or 50 µM Z-Leu-Leu-CHO (ZLL) (d). Scale bar: 250 µm. (B) Results expressed as a percentage of cells migrating in control conditions. Two-way ANOVA followed by a Bonferroni t-test: *P<0.05 vs control; n=6-15 independent experiments.





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