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Fig. 5. Migration of control and TRPC1-knockdown myoblasts. (A) Wound-healing migration assay of C2C12 myoblasts transfected with control shRNA (a) or TRPC1 shRNA (b). Scale bar: 200 µm. (B,C) Number of cells transfected with TRPC1 shRNA (B) and TRPC1 siRNA (C) that had migrated into the acellular area compared with control siRNA and shRNA transfected cells. *** P<0.001 vs control (in B, Student's t-test, n=9 independent experiments from three different cultures; in C, one-way ANOVA followed by a Student-Newman-Keuls test, n=6 experiments from three different cultures).