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Fig. 6. Calpain activity during differentiation of control and TRPC1-knockdown myoblasts. Calpain activity was measured as the rate of fluorescence increase of cells incubated with 10 µM Boc-Leu-Met-CMAC, a permeant and nonfluorescent substrate that, upon cleavage by calpain, becomes fluorescent. Calpain activity was measured at different stages of differentiation (day 0 to day 4) and all measurements were expressed relative to the calpain activity measured in control myoblasts at day 0 of differentiation, during the same session of measurements (n=10 or more controls per session). Two-way ANOVA followed by a Student-Newmann-Keuls test: **P<0.01 for controls at D1 vs control at D0, D2, D3 and D4; 
P<0.05 for TRPC1-knockdown at D1 vs control cells at D1; n=12-48 measurements from four different cultures.
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