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Figure 7


Fig. 7. Mn2+ influxes in control and in TRPC1-knockdown myoblasts. (A-C) The rate of quenching of Fura-PE3 by Mn2+ was used to estimate the influx of Ca2+ at different stages of differentiation (from day 0 to day 4) in control C2C12 myoblasts (A), in control shRNA (B, black columns) and TRPC1 shRNA-transfected cells (B, grey columns) and in control siRNA (C, black columns) and TRPC1 siRNA-transfected cells (C, grey columns). Statistical analysis: in A, one-way ANOVA, **P<0.01 for D1 vs D0, D2, D3 and D4; in B,C, two-way ANOVA followed by Student-Newmann-Keuls tests, *P<0.05 D1 control vs control at D0 and D2; §P<0.05 TRPC1-knockdown myoblasts at D1 vs controls at D1.





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