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Figure 4


Fig. 4. PI3K activity is required for BMP2-induced actin reorganization and cell migration in C2C12 cells. (A) Cells were serum-starved for 16 hours prior to preincubation with 15 µM LY294002 for 60 minutes and then stimulated with 3 nM BMP2 for the indicated times. Cell lysates were analyzed by immunoblotting with anti-Akt-P(Ser473) and reprobed with anti-Akt total antibody. (B) Cells treated as above were fixed and stained with TRITC-conjugated phalloidin. Scale bar: 20 µm. Cells with actin protrusions were quantified as shown in graph below. Mean ± s.e.m. from four independent experiments (*P<0.001, paired t-test). (C) Cell monolayers were wounded and allowed to migrate for 12 hours in the presence or absence of 3 nM BMP2 and/or LY294002. The graph in lower panel indicates the invaded area. Mean ± s.e.m. from three independent experiments (*P<0.001, paired t-test).





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